Genome sequence variation in the constricta strain dramatically alters the protein interaction and localization map of Potato yellow dwarf virus

Chanyong Jang, Renyuan Wang, Joseph Wells, Fabian Leon, Mark Farman, John Hammond, Michael M. Goodin

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The genome sequence of the constricta strain of Potato yellow dwarf virus (CYDV) was determined to be 12 792 nt long and organized into seven ORFs with the gene order 3'-N-X-P-Y-M-G-L-5', which encodes the nucleocapsid, phospho, movement, matrix, glyco, and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. Cloned ORFs for each gene, except L, were used to construct a protein interaction and localization map (PILM) for this virus, which shares greater than 80 % amino acid similarity in all ORFs except X and P with the sanguinolenta strain of this species (SYDV). Protein localization patterns and interactions unique to each viral strain were identified, resulting in strain-specific PILMs. Localization of CYDV and SYDV proteins in virus-infected cells mapped subcellular loci likely to be sites of replication, morphogenesis and movement.

Original languageEnglish
Article number000771
Pages (from-to)1526-1536
Number of pages11
JournalJournal of General Virology
Volume98
Issue number6
DOIs
StatePublished - Jun 2017

Bibliographical note

Publisher Copyright:
© 2017, Microbiology Society. All rights reserved.

Keywords

  • BiFC
  • Confocal
  • GFP
  • Interactome
  • Localization
  • Nicotiana benthamiana
  • Nuclear localization
  • Rhabdovirus

ASJC Scopus subject areas

  • Virology

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