TY - JOUR
T1 - Genome sequence variation in the constricta strain dramatically alters the protein interaction and localization map of Potato yellow dwarf virus
AU - Jang, Chanyong
AU - Wang, Renyuan
AU - Wells, Joseph
AU - Leon, Fabian
AU - Farman, Mark
AU - Hammond, John
AU - Goodin, Michael M.
N1 - Publisher Copyright:
© 2017, Microbiology Society. All rights reserved.
PY - 2017/6
Y1 - 2017/6
N2 - The genome sequence of the constricta strain of Potato yellow dwarf virus (CYDV) was determined to be 12 792 nt long and organized into seven ORFs with the gene order 3'-N-X-P-Y-M-G-L-5', which encodes the nucleocapsid, phospho, movement, matrix, glyco, and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. Cloned ORFs for each gene, except L, were used to construct a protein interaction and localization map (PILM) for this virus, which shares greater than 80 % amino acid similarity in all ORFs except X and P with the sanguinolenta strain of this species (SYDV). Protein localization patterns and interactions unique to each viral strain were identified, resulting in strain-specific PILMs. Localization of CYDV and SYDV proteins in virus-infected cells mapped subcellular loci likely to be sites of replication, morphogenesis and movement.
AB - The genome sequence of the constricta strain of Potato yellow dwarf virus (CYDV) was determined to be 12 792 nt long and organized into seven ORFs with the gene order 3'-N-X-P-Y-M-G-L-5', which encodes the nucleocapsid, phospho, movement, matrix, glyco, and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. Cloned ORFs for each gene, except L, were used to construct a protein interaction and localization map (PILM) for this virus, which shares greater than 80 % amino acid similarity in all ORFs except X and P with the sanguinolenta strain of this species (SYDV). Protein localization patterns and interactions unique to each viral strain were identified, resulting in strain-specific PILMs. Localization of CYDV and SYDV proteins in virus-infected cells mapped subcellular loci likely to be sites of replication, morphogenesis and movement.
KW - BiFC
KW - Confocal
KW - GFP
KW - Interactome
KW - Localization
KW - Nicotiana benthamiana
KW - Nuclear localization
KW - Rhabdovirus
UR - http://www.scopus.com/inward/record.url?scp=85023612148&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85023612148&partnerID=8YFLogxK
U2 - 10.1099/jgv.0.000771
DO - 10.1099/jgv.0.000771
M3 - Article
C2 - 28635588
AN - SCOPUS:85023612148
SN - 0022-1317
VL - 98
SP - 1526
EP - 1536
JO - Journal of General Virology
JF - Journal of General Virology
IS - 6
M1 - 000771
ER -