The genome sequence of the constricta strain of Potato yellow dwarf virus (CYDV) was determined to be 12 792 nt long and organized into seven ORFs with the gene order 3'-N-X-P-Y-M-G-L-5', which encodes the nucleocapsid, phospho, movement, matrix, glyco, and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. Cloned ORFs for each gene, except L, were used to construct a protein interaction and localization map (PILM) for this virus, which shares greater than 80 % amino acid similarity in all ORFs except X and P with the sanguinolenta strain of this species (SYDV). Protein localization patterns and interactions unique to each viral strain were identified, resulting in strain-specific PILMs. Localization of CYDV and SYDV proteins in virus-infected cells mapped subcellular loci likely to be sites of replication, morphogenesis and movement.
|Number of pages||11|
|Journal||Journal of General Virology|
|State||Published - Jun 2017|
Bibliographical noteFunding Information:
This research was supported, in part, by grants 0749519 from the U.S. National Science Foundation and KSEF-3490-RDE-019 from the Kentucky Science and Energy Foundation, awarded to M. M. G.
© 2017, Microbiology Society. All rights reserved.
- Nicotiana benthamiana
- Nuclear localization
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