Genome-wide determination of poly(A) site choice in plants

Pratap Kumar Pati; Liuyin Ma; Arthur G. Hunt

doi:10.1007/978-1-4939-2175-1_14

Genome-wide determination of poly(A) site choice in plants

Pratap Kumar Pati, Liuyin Ma, Arthur G. Hunt

Plant and Soil Sciences

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3′-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.

Original language	English
Pages (from-to)	159-174
Number of pages	16
Journal	Methods in Molecular Biology
Volume	1255
DOIs	https://doi.org/10.1007/978-1-4939-2175-1_14
State	Published - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

Alternative polyadenylation
Gene expression
High-throughput sequencing
PAT-seq

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2175-1_14

Cite this

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abstract = "Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3′-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.",

keywords = "Alternative polyadenylation, Gene expression, High-throughput sequencing, PAT-seq",

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doi = "10.1007/978-1-4939-2175-1_14",

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AU - Ma, Liuyin

AU - Hunt, Arthur G.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3′-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.

AB - Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3′-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.

KW - Alternative polyadenylation

KW - Gene expression

KW - High-throughput sequencing

KW - PAT-seq

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EP - 174

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Genome-wide determination of poly(A) site choice in plants

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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