Genome-wide determination of poly(A) site choice in plants

Pratap Kumar Pati; Liuyin Ma; Arthur G. Hunt

doi:10.1007/978-1-4939-2175-1_14

Genome-wide determination of poly(A) site choice in plants

Pratap Kumar Pati, Liuyin Ma, Arthur G. Hunt

Plant and Soil Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3'-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.

Original language	English
Title of host publication	Polyadenylation in Plants
Subtitle of host publication	Methods and Protocols
Pages	159-174
Number of pages	16
ISBN (Electronic)	9781493921751
DOIs	https://doi.org/10.1007/978-1-4939-2175-1_14
State	Published - Dec 8 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015. All rights are reserved.

Keywords

Alternative polyadenylation
Gene expression
High-throughput sequencing
PAT-seq

ASJC Scopus subject areas

General Agricultural and Biological Sciences
General Medicine
General Biochemistry, Genetics and Molecular Biology

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10.1007/978-1-4939-2175-1_14

Cite this

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AB - Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3'-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.

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KW - Gene expression

KW - High-throughput sequencing

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Genome-wide determination of poly(A) site choice in plants

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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