Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3'-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.
|Title of host publication||Polyadenylation in Plants|
|Subtitle of host publication||Methods and Protocols|
|Number of pages||16|
|State||Published - Dec 8 2014|
Bibliographical notePublisher Copyright:
© Springer Science+Business Media New York 2015. All rights are reserved.
- Alternative polyadenylation
- Gene expression
- High-throughput sequencing
ASJC Scopus subject areas
- Agricultural and Biological Sciences (all)
- Medicine (all)
- Biochemistry, Genetics and Molecular Biology (all)