Genome-Wide Identification of SnRK1 Catalytic α Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses

Chao Yang; Guangyu Shi; Yuping Li; Ming Luo; Hongxia Wang; Jihua Wang; Ling Yuan; Ying Wang; Yongqing Li

doi:10.3390/ijms24010121

Genome-Wide Identification of SnRK1 Catalytic α Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses

Chao Yang, Guangyu Shi, Yuping Li, Ming Luo, Hongxia Wang, Jihua Wang, Ling Yuan, Ying Wang, Yongqing Li

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Sucrose non-fermenting-1-related protein kinase-1 (SnRK1) and its scaffolding proteins, FCS-like zinc finger proteins (FLZs), are well conserved in land plants and involved in various processes of plant growth and stress responses. Glycyrrhiza inflata Bat. is a widely used licorice species with strong abiotic stress resistance, in which terpenoids and flavonoids are the major bioactive components. Here, we identified 2 SnRK1 catalytic α subunit encoding genes (GiSnRK1α1 and GiSnRK1α2) and 21 FLZ genes in G. inflata. Polygenetic analysis showed that the 21 GiFLZs could be divided into three groups. A total of 10 representative GiFLZ proteins interact with GiSnRK1α1, and they display overlapped subcellular localization (mainly in the nucleus and the cytoplasm) when transiently expressed in Nicotiana benthamiana leaf cells. Coinciding with the existence of various phytohormone-responsive and stress-responsive cis-regulatory elements in the GiSnRK1α and GiFLZ gene promoters, GiFLZs are actively responsive to methyl jasmonic acid (MeJA) and abscisic acid (ABA) treatments, and several GiFLZs and GiSnRK1α1 are regulated by drought and saline-alkaline stresses. Interestingly, GiSnRK1α and 20 of 21 GiFLZs (except for GiFLZ2) show higher expression in the roots than in the leaves. These data provide comprehensive information on the SnRK1 catalytic α subunit and the FLZ proteins in licorice for future functional characterization.

Original language	English
Article number	121
Journal	International Journal of Molecular Sciences
Volume	24
Issue number	1
DOIs	https://doi.org/10.3390/ijms24010121
State	Published - Jan 2023

Bibliographical note

Publisher Copyright:
© 2022 by the authors.

Funding

This research was funded by the Guangdong Basic and Applied Basic Research Foundation (2022A1515012319) and the Department of Science and Technology of Guangzhou (202002030442), the Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams (2020KJ148), the Foundation of the Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement (KF202203), the South China Botanical Garden, the Chinese Academy of Sciences (QNXM-02), and the Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden (PFGR202202).

Funders	Funder number
Department of Science and Technology of Guangzhou	202002030442
Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams	2020KJ148, KF202203
Shanghai Chenshan Botanical Garden	PFGR202202
South China Botanical Garden
Chinese Academy of Sciences	QNXM-02
Shanghai Key Laboratory of Plant Functional Genomics and Resources
Basic and Applied Basic Research Foundation of Guangdong Province	2022A1515012319

Keywords

FLZ
Glycyrrhiza inflata
SnRK1
growth
stress response

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/ijms24010121

Cite this

Yang, C., Shi, G., Li, Y., Luo, M., Wang, H., Wang, J., Yuan, L., Wang, Y., & Li, Y. (2023). Genome-Wide Identification of SnRK1 Catalytic α Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses. International Journal of Molecular Sciences, 24(1), Article 121. https://doi.org/10.3390/ijms24010121

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title = "Genome-Wide Identification of SnRK1 Catalytic α Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses",

abstract = "Sucrose non-fermenting-1-related protein kinase-1 (SnRK1) and its scaffolding proteins, FCS-like zinc finger proteins (FLZs), are well conserved in land plants and involved in various processes of plant growth and stress responses. Glycyrrhiza inflata Bat. is a widely used licorice species with strong abiotic stress resistance, in which terpenoids and flavonoids are the major bioactive components. Here, we identified 2 SnRK1 catalytic α subunit encoding genes (GiSnRK1α1 and GiSnRK1α2) and 21 FLZ genes in G. inflata. Polygenetic analysis showed that the 21 GiFLZs could be divided into three groups. A total of 10 representative GiFLZ proteins interact with GiSnRK1α1, and they display overlapped subcellular localization (mainly in the nucleus and the cytoplasm) when transiently expressed in Nicotiana benthamiana leaf cells. Coinciding with the existence of various phytohormone-responsive and stress-responsive cis-regulatory elements in the GiSnRK1α and GiFLZ gene promoters, GiFLZs are actively responsive to methyl jasmonic acid (MeJA) and abscisic acid (ABA) treatments, and several GiFLZs and GiSnRK1α1 are regulated by drought and saline-alkaline stresses. Interestingly, GiSnRK1α and 20 of 21 GiFLZs (except for GiFLZ2) show higher expression in the roots than in the leaves. These data provide comprehensive information on the SnRK1 catalytic α subunit and the FLZ proteins in licorice for future functional characterization.",

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author = "Chao Yang and Guangyu Shi and Yuping Li and Ming Luo and Hongxia Wang and Jihua Wang and Ling Yuan and Ying Wang and Yongqing Li",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2023",

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doi = "10.3390/ijms24010121",

language = "English",

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Genome-Wide Identification of SnRK1 Catalytic α Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses. / Yang, Chao; Shi, Guangyu; Li, Yuping et al.
In: International Journal of Molecular Sciences, Vol. 24, No. 1, 121, 01.2023.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Genome-Wide Identification of SnRK1 Catalytic α Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses

AU - Yang, Chao

AU - Shi, Guangyu

AU - Li, Yuping

AU - Luo, Ming

AU - Wang, Hongxia

AU - Wang, Jihua

AU - Yuan, Ling

AU - Wang, Ying

AU - Li, Yongqing

PY - 2023/1

Y1 - 2023/1

N2 - Sucrose non-fermenting-1-related protein kinase-1 (SnRK1) and its scaffolding proteins, FCS-like zinc finger proteins (FLZs), are well conserved in land plants and involved in various processes of plant growth and stress responses. Glycyrrhiza inflata Bat. is a widely used licorice species with strong abiotic stress resistance, in which terpenoids and flavonoids are the major bioactive components. Here, we identified 2 SnRK1 catalytic α subunit encoding genes (GiSnRK1α1 and GiSnRK1α2) and 21 FLZ genes in G. inflata. Polygenetic analysis showed that the 21 GiFLZs could be divided into three groups. A total of 10 representative GiFLZ proteins interact with GiSnRK1α1, and they display overlapped subcellular localization (mainly in the nucleus and the cytoplasm) when transiently expressed in Nicotiana benthamiana leaf cells. Coinciding with the existence of various phytohormone-responsive and stress-responsive cis-regulatory elements in the GiSnRK1α and GiFLZ gene promoters, GiFLZs are actively responsive to methyl jasmonic acid (MeJA) and abscisic acid (ABA) treatments, and several GiFLZs and GiSnRK1α1 are regulated by drought and saline-alkaline stresses. Interestingly, GiSnRK1α and 20 of 21 GiFLZs (except for GiFLZ2) show higher expression in the roots than in the leaves. These data provide comprehensive information on the SnRK1 catalytic α subunit and the FLZ proteins in licorice for future functional characterization.

AB - Sucrose non-fermenting-1-related protein kinase-1 (SnRK1) and its scaffolding proteins, FCS-like zinc finger proteins (FLZs), are well conserved in land plants and involved in various processes of plant growth and stress responses. Glycyrrhiza inflata Bat. is a widely used licorice species with strong abiotic stress resistance, in which terpenoids and flavonoids are the major bioactive components. Here, we identified 2 SnRK1 catalytic α subunit encoding genes (GiSnRK1α1 and GiSnRK1α2) and 21 FLZ genes in G. inflata. Polygenetic analysis showed that the 21 GiFLZs could be divided into three groups. A total of 10 representative GiFLZ proteins interact with GiSnRK1α1, and they display overlapped subcellular localization (mainly in the nucleus and the cytoplasm) when transiently expressed in Nicotiana benthamiana leaf cells. Coinciding with the existence of various phytohormone-responsive and stress-responsive cis-regulatory elements in the GiSnRK1α and GiFLZ gene promoters, GiFLZs are actively responsive to methyl jasmonic acid (MeJA) and abscisic acid (ABA) treatments, and several GiFLZs and GiSnRK1α1 are regulated by drought and saline-alkaline stresses. Interestingly, GiSnRK1α and 20 of 21 GiFLZs (except for GiFLZ2) show higher expression in the roots than in the leaves. These data provide comprehensive information on the SnRK1 catalytic α subunit and the FLZ proteins in licorice for future functional characterization.

KW - FLZ

KW - Glycyrrhiza inflata

KW - SnRK1

KW - growth

KW - stress response

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DO - 10.3390/ijms24010121

M3 - Article

C2 - 36613561

AN - SCOPUS:85145965291

SN - 1661-6596

VL - 24

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 1

M1 - 121

ER -

Genome-Wide Identification of SnRK1 Catalytic α Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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