Abstract
Histone modifications and histone variants barcode the genome and play major roles in epigenetic regulations. Chromatin immunoprecipitation (ChIP) coupled with next-generation sequencing (NGS) is a well-established method to investigate the landscape of epigenetic marks at a genomic level. Here, we describe procedures for conducting ChIP, subsequent NGS library construction, and data analysis on histone modifications and histone variants in Arabidopsis thaliana. We also describe an optimized nuclear isolation procedure to prepare chromatin for ChIP in the liverwort, Marchantia polymorpha, which is the emerging model plant ideal for evolutionary studies.
Original language | English |
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Title of host publication | Methods in Molecular Biology |
Pages | 93-106 |
Number of pages | 14 |
DOIs | |
State | Published - 2017 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 1610 |
ISSN (Print) | 1064-3745 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media LLC 2017.
Funding
Thanks to S. Akimcheva and J. S. Russell for technical assistance and critical reading, respectively. AO was supported by a Grant-in-Aid for the Japan Society for the Promotion of Science (JSPS) Fellows. RY, TK, EA, and FB were funded by GMI and FWF (grants 2163-B16 linked to ERA-CAPS project EVOREPRO).
Funders | Funder number |
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GMI | |
Japan Society for the Promotion of Science | |
Austrian Science Fund/FWF | 2163-B16 |
Austrian Science Fund/FWF |
Keywords
- Arabidopsis thaliana
- Chromatin immunoprecipitation
- Histone modifications
- Histone variants
- Marchantia polymorpha
ASJC Scopus subject areas
- Molecular Biology
- Genetics