Genome-wide screens for identification of host factors in viral replication

Tadas Panavas, Elena Serviene, Judit Pogany, Peter D. Nagy

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

14 Scopus citations

Abstract

The central step in virus infection cycle is replication, which depends on viral and host factors. Model hosts, such as yeast, can be very valuable to identify host factors and study the functional interactions of host factors with viral proteins and/or the virus nucleic acids. The advantages of using yeast include the availability of (i) single gene-deletion library, (ii) the essential gene library (yTHC), (iii) the controllable small or large-scale expression of viral proteins and nucleic acids, and (iv) the rapid growth of yeast strains. Here, we describe procedures, which facilitate high-throughput analysis of tombusvirus replication in yeast.

Original languageEnglish
Title of host publicationPlant Virology Protocols
Subtitle of host publicationFrom Viral Sequence to Protein Function
Pages615-624
Number of pages10
DOIs
StatePublished - 2008

Publication series

NameMethods in Molecular Biology
Volume451
ISSN (Print)1064-3745

Keywords

  • High-throughput transformation
  • High-throughput viral RNA extraction
  • Host factors
  • Northern blotting
  • Tombusviruses
  • Yeast

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Genome-wide screens for identification of host factors in viral replication'. Together they form a unique fingerprint.

Cite this