Development of next-generation sequencing, coupled with the advancement of computational methods, has allowed researchers to access the transcriptomes of recalcitrant genomes such as those of medicinal plant species. Through the sequencing of even a few cDNA libraries, a broad representation of the transcriptome of any medicinal plant species can be obtained, providing a robust resource for gene discovery and downstream biochemical pathway discovery. When coupled to estimation of expression abundances in specific tissues from a developmental series, biotic stress, abiotic stress, or elicitor challenge, informative coexpression and differential expression estimates on a whole transcriptome level can be obtained to identify candidates for function discovery.
|Title of host publication||Natural Product Biosynthesis by Microorganisms and Plants, Part C|
|Number of pages||21|
|State||Published - 2012|
|Name||Methods in Enzymology|
Bibliographical noteFunding Information:
Funding for medicinal plant transcriptome work was provided by a grant to J. C., D. D. P., and C. R. B. from the National Institute of General Medical Sciences (1RC2GM092521) and from the Michigan State University GREEEN program to C. R. B. (GR11-181).
- Expression abundance
ASJC Scopus subject areas
- Molecular Biology