GFP fluorescence reports Period1 circadian gene regulation in the mammalian biological clock

Sandra J. Kuhlman, Jorge E. Quintero, Douglas G. McMahon

Research output: Contribution to journalArticlepeer-review

110 Scopus citations

Abstract

Endogenous cyclic activation of a specific set of genes, including Period1 (Per1), drive circadian rhythms in the suprachiasmatic nucleus (SCN), a biological clock nucleus of the brain. We have produced transgenic mice in which a degradable form of recombinant jellyfish green fluorescent protein (GFP) is driven by the mouse Period1 (mPer1) gene promoter. GFP protein is expressed in the circadian neural structures of the retina and SCN. Fluorescent signals are resolved at the level of individual neurons, mPer1- driven GFP fluorescence intensity reports light-induction and circadian rhythmicity in SCN neurons. This circadian reporter transgene captures the gene expression dynamics of living biological clock neurons and ensembles, providing a novel view of this brain function. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)1479-1482
Number of pages4
JournalNeuroReport
Volume11
Issue number7
DOIs
StatePublished - May 15 2000

Keywords

  • Circadian rhythm
  • Fluorescence
  • Green Fluorescent Protein
  • Period1
  • Reporter
  • Suprachiasmatic nucleus
  • Transgene

ASJC Scopus subject areas

  • Neuroscience (all)

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