Global Genomics and Proteomics Approaches to Identify Host Factors as Targets to Induce Resistance Against Tomato Bushy Stunt Virus

Peter D. Nagy, Judit Pogany

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

85 Scopus citations

Abstract

The success of RNA viruses as pathogens of plants, animals, and humans depends on their ability to reprogram the host cell metabolism to support the viral infection cycle and to suppress host defense mechanisms. Plus-strand (+)RNA viruses have limited coding potential necessitating that they co-opt an unknown number of host factors to facilitate their replication in host cells. Global genomics and proteomics approaches performed with Tomato bushy stunt virus (TBSV) and yeast (Saccharomyces cerevisiae) as a model host have led to the identification of 250 host factors affecting TBSV RNA replication and recombination or bound to the viral replicase, replication proteins, or the viral RNA. The roles of a dozen host factors involved in various steps of the replication process have been validated in yeast as well as a plant host. Altogether, the large number of host factors identified and the great variety of cellular functions performed by these factors indicate the existence of a truly complex interaction between TBSV and the host cell. This review summarizes the advantages of using a simple plant virus and yeast as a model host to advance our understanding of virus–host interactions at the molecular and cellular levels. The knowledge of host factors gained can potentially be used to inhibit virus replication via gene silencing, expression of dominant negative mutants, or design of specific chemical inhibitors leading to novel specific or broad-range resistance and antiviral tools against (+)RNA plant viruses.

Original languageEnglish
Title of host publicationAdvances in Virus Research
Pages123-177
Number of pages55
DOIs
StatePublished - Jan 2010

Publication series

NameAdvances in Virus Research
Volume76
ISSN (Print)0065-3527
ISSN (Electronic)1557-8399

Bibliographical note

Publisher Copyright:
© 2010 Elsevier Inc.

Keywords

  • Genome-wide screens
  • Host factors
  • Protein–protein interaction
  • RNA–protein interaction
  • Viral replicase complex
  • Virus replication
  • Yeast as a host

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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