Glyoxylate regeneration pathway in the methylotroph Methylobacterium extorquens AM1

Natalia Korotkova, Ludmila Chistoserdova, Vladimir Kuksa, Mary E. Lidstrom

Research output: Contribution to journalArticlepeer-review

88 Scopus citations


Most serine cycle methylotrophic bacteria lack isocitrate lyase and convert acetyl coenzyme A (acetyl-CoA) to glyoxylate via a novel pathway thought to involve butyryl-CoA and propionyl-CoA as intermediates. In this study we have used a genome analysis approach followed by mutation to test a number of genes for involvement in this novel pathway. We show that methylmalonyl-CoA mutase, an R-specific crotonase, isobutyryl-CoA dehydrogenase, and a GTPase are involved in glyoxylate regeneration. We also monitored the fate of 14C-labeled carbon originating from acetate, butyrate, or bicarbonate in mutants defective in glyoxylate regeneration and identified new potential intermediates in the pathway: ethylmalonyl-CoA, methylsuccinyl-CoA, isobutyryl-CoA, methacrylyl-CoA, and β-hydroxyisobutyryl-CoA. A new scheme for the pathway is proposed based on these data.

Original languageEnglish
Pages (from-to)1750-1758
Number of pages9
JournalJournal of Bacteriology
Issue number6
StatePublished - 2002

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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