TY - JOUR
T1 - Guided bone regeneration with or without a collagen membrane in rats with induced diabetes mellitus
T2 - Histomorphometric and immunolocalization analysis of angiogenesis and bone turnover markers
AU - Jardini, Maria Aparecida Neves
AU - Tera, Tábata de Mello
AU - Meyer, Augusto Cesar de Andrade
AU - Moretto, Camilla Magnoni
AU - do Prado, Renata Falchete
AU - Santamaria, Mauro Pedrine
N1 - Publisher Copyright:
© 2016 by Quintessence Publishing Co Inc.
PY - 2016
Y1 - 2016
N2 - Purpose: Diabetes mellitus (DM) affects the processes of repair, wound healing, and bone remodeling. This study was conducted to evaluate autologous bone graft integration, either with or without guided bone regeneration, through analyzing the expression of bone reabsorption markers and neovascularization in rats suffering from DM. Materials and Methods: Thirty adult Wistar rats were divided into two groups: The DM group received an injection of alloxan monohydrate (150 mg/kg), and the control group received an injection of sterile saline. Fifteen days afterward, an autologous bone grafting was performed in each of their arches, with the insertion of a membrane into the left arch. Euthanasia occurred in 7, 21, or 60 days after the surgery. Bone samples were processed for histomorphometric and immunohistochemical analyses. Results: After a statistical analysis of the data, the presence of DM did not interfere negatively in the bone autograft repair. The collagen membrane favored the graft integration into the recipient bed and the bone neoformation around the graft. Greater vascularization was observed between 21 and 60 days after the surgery, which increased bone formation and resulted in the graft integration. Only the RANK marker showed a significant difference in the glycemic groups. Transglutaminase 2 was significant for the membrane presence and experimental time. Conclusion: It is hence concluded that diabetes mellitus does not interfere with bone reabsorption via the RANK/RANKL/OPG. The graft integration was similar between the groups; however, the results of hyperglycemia with the collagen membrane indicate greater bone growth after graft placement.
AB - Purpose: Diabetes mellitus (DM) affects the processes of repair, wound healing, and bone remodeling. This study was conducted to evaluate autologous bone graft integration, either with or without guided bone regeneration, through analyzing the expression of bone reabsorption markers and neovascularization in rats suffering from DM. Materials and Methods: Thirty adult Wistar rats were divided into two groups: The DM group received an injection of alloxan monohydrate (150 mg/kg), and the control group received an injection of sterile saline. Fifteen days afterward, an autologous bone grafting was performed in each of their arches, with the insertion of a membrane into the left arch. Euthanasia occurred in 7, 21, or 60 days after the surgery. Bone samples were processed for histomorphometric and immunohistochemical analyses. Results: After a statistical analysis of the data, the presence of DM did not interfere negatively in the bone autograft repair. The collagen membrane favored the graft integration into the recipient bed and the bone neoformation around the graft. Greater vascularization was observed between 21 and 60 days after the surgery, which increased bone formation and resulted in the graft integration. Only the RANK marker showed a significant difference in the glycemic groups. Transglutaminase 2 was significant for the membrane presence and experimental time. Conclusion: It is hence concluded that diabetes mellitus does not interfere with bone reabsorption via the RANK/RANKL/OPG. The graft integration was similar between the groups; however, the results of hyperglycemia with the collagen membrane indicate greater bone growth after graft placement.
KW - Angiogenesis
KW - Bone reabsorption
KW - Diabetes mellitus
KW - Guided bone regeneration
KW - RANK/RANKL/ OPG
KW - Transglutaminase 2
KW - VEGF
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U2 - 10.11607/jomi.4358
DO - 10.11607/jomi.4358
M3 - Article
C2 - 27447161
AN - SCOPUS:84985911322
SN - 0882-2786
VL - 31
SP - 918
EP - 927
JO - International Journal of Oral and Maxillofacial Implants
JF - International Journal of Oral and Maxillofacial Implants
IS - 4
ER -