HDAC6 Mediates Macrophage iNOS Expression and Excessive Nitric Oxide Production in the Blood During Endotoxemia

Yan Wang, Ke Wang, Jian Fu

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Excessive nitric oxide (NO) production and NO-mediated nitrative stress contribute to vascular dysfunction, inflammation, and tissue injury in septic shock. New therapeutic targets are urgently needed to provide better control of NO level during septic shock. In the present study, we investigated the role of HDAC6 in the regulation of NO production and nitrative stress in a mouse model of endotoxin-induced septic shock. HDAC6 deficient mice and a specific HDAC6 inhibitor were utilized in our studies. Our data clearly indicate that HDAC6 is an important mediator of NO production in macrophages. HDAC6 mediates NO production through the regulation of iNOS expression in macrophages. HDAC6 up-regulates iNOS expression in macrophages by modulating STAT1 activation and IRF-1 expression. HDAC6 inhibition potently blocked endotoxin-induced STAT1 activation and iNOS expression in macrophages. Furthermore, HDAC6 contributes to excessive NO production and nitrotyrosine level in the blood and promotes iNOS expression in the lung tissues during septic shock. Our data reveal a novel HDAC6/STAT1/iNOS pathway that mediates excessive NO production and nitrative stress in septic shock.

Original languageEnglish
Article number1893
JournalFrontiers in Immunology
Volume11
DOIs
StatePublished - Aug 20 2020

Bibliographical note

Publisher Copyright:
© Copyright © 2020 Wang, Wang and Fu.

Keywords

  • inflammation
  • macrophage
  • microtubule
  • nitric oxide
  • sepsis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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