TY - JOUR
T1 - HDL remodeling during the acute phase response
AU - Jahangiri, Anisa
AU - De Beer, Maria C.
AU - Noffsinger, Victoria
AU - Tannock, Lisa R.
AU - Ramaiah, Chandrashekar
AU - Webb, Nancy R.
AU - Van Der Westhuyzen, Deneys R.
AU - De Beer, Frederick C.
PY - 2009/2
Y1 - 2009/2
N2 - Objective - The purpose of this study was to examine the interactive action of serum amyloid A (SAA), group IIA secretory phospholipase A2 (sPLA2-IIA), and cholesteryl ester transfer protein (CETP) on HDL remodeling and cholesterol efflux during the acute phase (AP) response elicited in humans after cardiac surgery. Methods and Results - Plasma was collected from patients before (pre-AP), 24 hours after (AP-1 d), and 5 days after cardiac surgery (AP-5 d). SAA levels were increased 16-fold in AP-1 d samples. The activity of sPLA2-IIA was increased from 77.7±38.3 U/mL (pre-AP) to 281.4±57.1 U/mL (AP-1 d; P<0.001). CETP mass and activity reduction was commensurate to the reduction of HDL cholesterol levels. The combined action of SAA, sPLA2-IIA, and CETP in vitro markedly remodeled HDL with the generation of lipid-poor apoA-I from both pre-AP and AP-1 d HDL. The net result of this remodeling was a relative preservation of ABCA1- and ABCG1-dependent cholesterol efflux during the acute phase response. Conclusions - Our results show that the many and complex changes in plasma proteins during the acute phase response markedly remodel HDL with functional implications, particularly the relative retention of cholesterol efflux capacity.
AB - Objective - The purpose of this study was to examine the interactive action of serum amyloid A (SAA), group IIA secretory phospholipase A2 (sPLA2-IIA), and cholesteryl ester transfer protein (CETP) on HDL remodeling and cholesterol efflux during the acute phase (AP) response elicited in humans after cardiac surgery. Methods and Results - Plasma was collected from patients before (pre-AP), 24 hours after (AP-1 d), and 5 days after cardiac surgery (AP-5 d). SAA levels were increased 16-fold in AP-1 d samples. The activity of sPLA2-IIA was increased from 77.7±38.3 U/mL (pre-AP) to 281.4±57.1 U/mL (AP-1 d; P<0.001). CETP mass and activity reduction was commensurate to the reduction of HDL cholesterol levels. The combined action of SAA, sPLA2-IIA, and CETP in vitro markedly remodeled HDL with the generation of lipid-poor apoA-I from both pre-AP and AP-1 d HDL. The net result of this remodeling was a relative preservation of ABCA1- and ABCG1-dependent cholesterol efflux during the acute phase response. Conclusions - Our results show that the many and complex changes in plasma proteins during the acute phase response markedly remodel HDL with functional implications, particularly the relative retention of cholesterol efflux capacity.
KW - CETP
KW - HDL
KW - Inflammation
KW - SAA
KW - apoA-I
UR - http://www.scopus.com/inward/record.url?scp=59449107512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59449107512&partnerID=8YFLogxK
U2 - 10.1161/ATVBAHA.108.178681
DO - 10.1161/ATVBAHA.108.178681
M3 - Article
C2 - 19008529
AN - SCOPUS:59449107512
SN - 1079-5642
VL - 29
SP - 261
EP - 267
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
IS - 2
ER -