Hello Darkness, My Old Friend: A Tutorial of Nanda-Hamner Protocols

Nicholas M. Teets, Megan E. Meuti

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Plants and animals use circadian and photoperiodic timekeeping mechanisms to respond to daily and seasonal changes in light:dark and appropriately coordinate their development. Although the mechanisms that may connect the circadian and photoperiodic clock are still unclear in many species, researchers have been using Nanda-Hamner protocols for decades to elucidate how seasonal time is measured and determine whether seasonal responses have a circadian basis in a given species. In this brief tutorial we describe how to design and interpret the results of Nanda-Hamner experiments, and provide suggestions on how to use both Nanda-Hamner protocols and modern molecular experiments to better understand the mechanisms of seasonal timekeeping.

Original languageEnglish
Pages (from-to)221-225
Number of pages5
JournalJournal of Biological Rhythms
Volume36
Issue number3
DOIs
StatePublished - Jun 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s).

Keywords

  • Nanda-Hamner protocol
  • circadian rhythms
  • external coincidence model
  • hourglass model
  • photoperiodism

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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