Herpes simplex virus type 1 recombination: The U(c)-DR1 region is required for high-level a-sequence-mediated recombination

R. E. Dutch, B. V. Zemelman, I. R. Lehman

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The a sequences of herpes simplex virus type 1 are believed to be the cis sites for inversion events that generate four isomeric forms of the viral genome. Using an assay that measures deletion of a β-galactosidase gene positioned between two directly repeated sequences in plasmids transiently maintained in Vero cells, we had found that the a sequence is more recombinogenic than another sequence of similar size. To investigate the basis for the enhanced recombination mediated by the a sequence, we examined plasmids containing direct repeats of approximately 350 bp from a variety of sources and with a wide range of G+C content. We observed that all of these plasmids show similar recombination frequencies (3 to 4%) in herpes simplex virus type 1-infected cells. However, recombination between directly repeated a sequences occurs at twice this frequency (6 to 10%). In addition, we find that insertion of a cleavage site for an a-sequence-specific endonuclease into the repeated sequences does not appreciably increase the frequency of recombination, indicating that the presence of endonuclease cleavage sites within the a sequence does not account for its recombinogenicity. Finally, by replacing segments of the a sequence with DNA fragments of similar length, we have determined that only the 95-bp U(c)-DR1 segment is indispensable for high-level a-sequence-mediated recombination.

Original languageEnglish
Pages (from-to)3733-3741
Number of pages9
JournalJournal of Virology
Volume68
Issue number6
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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