hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids

Yangfei Xiang; Yoshiaki Tanaka; Bilal Cakir; Benjamin Patterson; Kun Yong Kim; Pingnan Sun; Young Jin Kang; Mei Zhong; Xinran Liu; Prabir Patra; Sang Hun Lee; Sherman M. Weissman; In Hyun Park

doi:10.1016/j.stem.2018.12.015

hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids

Yangfei Xiang, Yoshiaki Tanaka, Bilal Cakir, Benjamin Patterson, Kun Yong Kim, Pingnan Sun, Young Jin Kang, Mei Zhong, Xinran Liu, Prabir Patra, Sang Hun Lee, Sherman M. Weissman, In Hyun Park

Neuroscience

Research output: Contribution to journal › Article › peer-review

224 Scopus citations

Abstract

Xiang and colleagues report a method for generating human thalamus-like brain organoids (hThOs) that recapitulate the development of human thalamus. By fusing hThOs and cortical-like brain organoids (hCOs), they establish a 3D system in a dish to create the reciprocal projections between thalamus and cortex.

Original language	English
Pages (from-to)	487-497.e7
Journal	Cell Stem Cell
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/j.stem.2018.12.015
State	Published - Mar 7 2019

Bibliographical note

Funding Information:
We thank Dr. Andrew G. Elefanty for sharing HES-3 NKX2-1 GFP/w human ES cell line. I.-H.P. was partly supported by NIH ( GM111667-01 , R01AA025080-01 , R01CA203011-2 ), CSCRF ( 14-SCC-YALE-01 , 16-RMB-YALE-04 ), Kavli Foundation , and KRIBB/KRCF research initiative program ( NAP-09-3 ). This work was supported by the College of Medicine, University of Arkansas for Medical Sciences to S.-H.L. and Core Facilities of the Center for Translational Neuroscience , Award P30 GM110702 from the IDeA program at NIGMS . Computation time was provided by Yale University Biomedical High Performance Computing Center .

Funding Information:
We thank Dr. Andrew G. Elefanty for sharing HES-3 NKX2-1GFP/w human ES cell line. I.-H.P. was partly supported by NIH (GM111667-01, R01AA025080-01, R01CA203011-2), CSCRF (14-SCC-YALE-01, 16-RMB-YALE-04), Kavli Foundation, and KRIBB/KRCF research initiative program (NAP-09-3). This work was supported by the College of Medicine, University of Arkansas for Medical Sciences to S.-H.L. and Core Facilities of the Center for Translational Neuroscience, Award P30 GM110702 from the IDeA program at NIGMS. Computation time was provided by Yale University Biomedical High Performance Computing Center.

Publisher Copyright:
© 2018 Elsevier Inc.

Keywords

brain organoid
cortex
corticothalamic
fusion
hESC
single cell RNA-seq
thalamic organoid
thalamocortical
thalamus

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

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10.1016/j.stem.2018.12.015

Cite this

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title = "hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids",

abstract = "Xiang and colleagues report a method for generating human thalamus-like brain organoids (hThOs) that recapitulate the development of human thalamus. By fusing hThOs and cortical-like brain organoids (hCOs), they establish a 3D system in a dish to create the reciprocal projections between thalamus and cortex.",

keywords = "brain organoid, cortex, corticothalamic, fusion, hESC, single cell RNA-seq, thalamic organoid, thalamocortical, thalamus",

author = "Yangfei Xiang and Yoshiaki Tanaka and Bilal Cakir and Benjamin Patterson and Kim, {Kun Yong} and Pingnan Sun and Kang, {Young Jin} and Mei Zhong and Xinran Liu and Prabir Patra and Lee, {Sang Hun} and Weissman, {Sherman M.} and Park, {In Hyun}",

note = "Funding Information: We thank Dr. Andrew G. Elefanty for sharing HES-3 NKX2-1 GFP/w human ES cell line. I.-H.P. was partly supported by NIH ( GM111667-01 , R01AA025080-01 , R01CA203011-2 ), CSCRF ( 14-SCC-YALE-01 , 16-RMB-YALE-04 ), Kavli Foundation , and KRIBB/KRCF research initiative program ( NAP-09-3 ). This work was supported by the College of Medicine, University of Arkansas for Medical Sciences to S.-H.L. and Core Facilities of the Center for Translational Neuroscience , Award P30 GM110702 from the IDeA program at NIGMS . Computation time was provided by Yale University Biomedical High Performance Computing Center . Funding Information: We thank Dr. Andrew G. Elefanty for sharing HES-3 NKX2-1GFP/w human ES cell line. I.-H.P. was partly supported by NIH (GM111667-01, R01AA025080-01, R01CA203011-2), CSCRF (14-SCC-YALE-01, 16-RMB-YALE-04), Kavli Foundation, and KRIBB/KRCF research initiative program (NAP-09-3). This work was supported by the College of Medicine, University of Arkansas for Medical Sciences to S.-H.L. and Core Facilities of the Center for Translational Neuroscience, Award P30 GM110702 from the IDeA program at NIGMS. Computation time was provided by Yale University Biomedical High Performance Computing Center. Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

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AU - Xiang, Yangfei

AU - Tanaka, Yoshiaki

AU - Cakir, Bilal

AU - Patterson, Benjamin

AU - Kim, Kun Yong

AU - Sun, Pingnan

AU - Kang, Young Jin

AU - Zhong, Mei

AU - Liu, Xinran

AU - Patra, Prabir

AU - Lee, Sang Hun

AU - Weissman, Sherman M.

AU - Park, In Hyun

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hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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