Heterogeneity of human serum antibody responses to P. gingivalis in periodontitis: Effects of age, race/ethnicity, and sex

J. L. Ebersole; M. Al-Sabbagh; D. R. Dawson

doi:10.1016/j.imlet.2019.12.004

Heterogeneity of human serum antibody responses to P. gingivalis in periodontitis: Effects of age, race/ethnicity, and sex

J. L. Ebersole, M. Al-Sabbagh, D. R. Dawson

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Aging humans display an increased prevalence and severity of periodontitis, although the mechanisms underlying these findings remain poorly understood. This report examined antigenic diversity of P. gingivalis related to disease presence and patient demographics. Serum IgG antibody to P. gingivalis strains ATCC33277, FDC381, W50 (ATCC53978), W83, A7A1-28 (ATCC53977) and A7436 was measured in 426 participants [periodontally healthy (n = 61), gingivitis (N = 66) or various levels of periodontitis (N = 299)]. We hypothesized that antigenic diversity in P. gingivalis could contribute to a lack of “immunity” in the chronic infections of periodontal disease. Across the strains, the antibody levels in the oldest age group were lower than in the youngest groups, and severe periodontitis patients did not show higher antibody with aging. While 80 % of the periodontitis patients in any age group showed an elevated response to at least one of the P. gingivalis strains, the patterns of individual responses in the older group were also substantially different than the other age groups. Significantly greater numbers of older patients showed strain-specific antibody profiles to only 1 strain. The findings support that P. gingivalis may demonstrate antigenic diversity/drift within patients and could be one factor to help explain the inefficiency/ineffectiveness of the adaptive immune response in managing the infection.

Original language	English
Pages (from-to)	11-21
Number of pages	11
Journal	Immunology Letters
Volume	218
DOIs	https://doi.org/10.1016/j.imlet.2019.12.004
State	Published - Feb 2020

Bibliographical note

Publisher Copyright:
© 2019 European Federation of Immunological Societies

Funding

We want to acknowledge the support of U.S.P.H.S . grant RR020145 and GM103538 to the Center for Biomedical Research Excellence, and funding from the Center for Oral Health Research at the University of Kentucky College of Dentistry . Also, expert technical assistance was provided by M.J. Steffen and Dr. R. Peyyala in providing the bacterial antigens and supporting the antibody measurements. The substantial contributions of the clinical support staff in the Delta Dental of Kentucky Clinical Research Center are also acknowledged. Appendix A We want to acknowledge the support of U.S.P.H.S. grant RR020145 and GM103538 to the Center for Biomedical Research Excellence, and funding from the Center for Oral Health Research at the University of Kentucky College of Dentistry. Also, expert technical assistance was provided by M.J. Steffen and Dr. R. Peyyala in providing the bacterial antigens and supporting the antibody measurements. The substantial contributions of the clinical support staff in the Delta Dental of Kentucky Clinical Research Center are also acknowledged.

Funders	Funder number
Center for Oral Health Research
University of Kentucky General Clinical Research Center
U.S.P.H.S.	GM103538, RR020145
National Center for Research Resources	P20RR020145
University of Kentucky College of Dentistry
Israeli Centers for Research Excellence

Keywords

Adaptive immunity
Aging
Antibody
Antigenic drift
Periodontitis

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.imlet.2019.12.004

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title = "Heterogeneity of human serum antibody responses to P. gingivalis in periodontitis: Effects of age, race/ethnicity, and sex",

abstract = "Aging humans display an increased prevalence and severity of periodontitis, although the mechanisms underlying these findings remain poorly understood. This report examined antigenic diversity of P. gingivalis related to disease presence and patient demographics. Serum IgG antibody to P. gingivalis strains ATCC33277, FDC381, W50 (ATCC53978), W83, A7A1-28 (ATCC53977) and A7436 was measured in 426 participants [periodontally healthy (n = 61), gingivitis (N = 66) or various levels of periodontitis (N = 299)]. We hypothesized that antigenic diversity in P. gingivalis could contribute to a lack of “immunity” in the chronic infections of periodontal disease. Across the strains, the antibody levels in the oldest age group were lower than in the youngest groups, and severe periodontitis patients did not show higher antibody with aging. While 80 % of the periodontitis patients in any age group showed an elevated response to at least one of the P. gingivalis strains, the patterns of individual responses in the older group were also substantially different than the other age groups. Significantly greater numbers of older patients showed strain-specific antibody profiles to only 1 strain. The findings support that P. gingivalis may demonstrate antigenic diversity/drift within patients and could be one factor to help explain the inefficiency/ineffectiveness of the adaptive immune response in managing the infection.",

keywords = "Adaptive immunity, Aging, Antibody, Antigenic drift, Periodontitis",

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AU - Al-Sabbagh, M.

AU - Dawson, D. R.

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N2 - Aging humans display an increased prevalence and severity of periodontitis, although the mechanisms underlying these findings remain poorly understood. This report examined antigenic diversity of P. gingivalis related to disease presence and patient demographics. Serum IgG antibody to P. gingivalis strains ATCC33277, FDC381, W50 (ATCC53978), W83, A7A1-28 (ATCC53977) and A7436 was measured in 426 participants [periodontally healthy (n = 61), gingivitis (N = 66) or various levels of periodontitis (N = 299)]. We hypothesized that antigenic diversity in P. gingivalis could contribute to a lack of “immunity” in the chronic infections of periodontal disease. Across the strains, the antibody levels in the oldest age group were lower than in the youngest groups, and severe periodontitis patients did not show higher antibody with aging. While 80 % of the periodontitis patients in any age group showed an elevated response to at least one of the P. gingivalis strains, the patterns of individual responses in the older group were also substantially different than the other age groups. Significantly greater numbers of older patients showed strain-specific antibody profiles to only 1 strain. The findings support that P. gingivalis may demonstrate antigenic diversity/drift within patients and could be one factor to help explain the inefficiency/ineffectiveness of the adaptive immune response in managing the infection.

AB - Aging humans display an increased prevalence and severity of periodontitis, although the mechanisms underlying these findings remain poorly understood. This report examined antigenic diversity of P. gingivalis related to disease presence and patient demographics. Serum IgG antibody to P. gingivalis strains ATCC33277, FDC381, W50 (ATCC53978), W83, A7A1-28 (ATCC53977) and A7436 was measured in 426 participants [periodontally healthy (n = 61), gingivitis (N = 66) or various levels of periodontitis (N = 299)]. We hypothesized that antigenic diversity in P. gingivalis could contribute to a lack of “immunity” in the chronic infections of periodontal disease. Across the strains, the antibody levels in the oldest age group were lower than in the youngest groups, and severe periodontitis patients did not show higher antibody with aging. While 80 % of the periodontitis patients in any age group showed an elevated response to at least one of the P. gingivalis strains, the patterns of individual responses in the older group were also substantially different than the other age groups. Significantly greater numbers of older patients showed strain-specific antibody profiles to only 1 strain. The findings support that P. gingivalis may demonstrate antigenic diversity/drift within patients and could be one factor to help explain the inefficiency/ineffectiveness of the adaptive immune response in managing the infection.

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Heterogeneity of human serum antibody responses to P. gingivalis in periodontitis: Effects of age, race/ethnicity, and sex

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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