TY - JOUR
T1 - High correlation in antibody titers between the Sabin-Feldman dye test and an enzyme-linked immunosorbent assay detecting immunoglobulin G antibodies to the nucleoside triphosphate hydrolase of Toxoplasma gondii
AU - Asai, T.
AU - Mizuno, F.
AU - Kojima, S.
AU - Takeuchi, T.
AU - Kobayashi, A.
AU - Suzuki, Y.
PY - 1992
Y1 - 1992
N2 - An enzyme-linked immunosorbent assay to detect immunoglobulin G antibodies against nucleoside triphosphate hydrolase, which is a specific and dominant antigen of Toxoplasma gondii, was developed, and the sensitivity and specificity of the test were compared with those of the Sabin-Feldman dye test. One hundred percent agreement was observed in comparative study between those tests on 37 positive and 50 negative human sera. Antibody titers in the enzyme-linked immunosorbent assay test, which were expressed as the reciprocal of the highest positive dilution of serum, were just 100 times those in the dye test on 81% (30 of 37) of the positive sera.
AB - An enzyme-linked immunosorbent assay to detect immunoglobulin G antibodies against nucleoside triphosphate hydrolase, which is a specific and dominant antigen of Toxoplasma gondii, was developed, and the sensitivity and specificity of the test were compared with those of the Sabin-Feldman dye test. One hundred percent agreement was observed in comparative study between those tests on 37 positive and 50 negative human sera. Antibody titers in the enzyme-linked immunosorbent assay test, which were expressed as the reciprocal of the highest positive dilution of serum, were just 100 times those in the dye test on 81% (30 of 37) of the positive sera.
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U2 - 10.1128/jcm.30.5.1291-1293.1992
DO - 10.1128/jcm.30.5.1291-1293.1992
M3 - Comment/debate
C2 - 1316368
AN - SCOPUS:0026548637
SN - 0095-1137
VL - 30
SP - 1291
EP - 1293
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 5
ER -