High levels of mitochondrial DNA deletions in skeletal muscle of old rhesus monkeys

Steven R. Schwarze; Connie M. Lee; Susan S. Chung; Ellen B. Roecker; Richard Weindruch; Judd M. Aiken

doi:10.1016/0047-6374(95)01611-3

High levels of mitochondrial DNA deletions in skeletal muscle of old rhesus monkeys

Steven R. Schwarze, Connie M. Lee, Susan S. Chung, Ellen B. Roecker, Richard Weindruch, Judd M. Aiken

Health and Clinical Sciences

Research output: Contribution to journal › Article › peer-review

113 Scopus citations

Abstract

Mitochondrial DNA (mtDNA) deletions increase in abundance with age in many tissues, however, their calculated low levels (usually < 0.1%) in samples from tissue homogenates containing thousands of cells argue against physiologic significance. Through the analysis of defined numbers of cells (skeletal muscle fibers) from rhesus monkeys, we report that the calculated abundance of specific mtDNA deletions is dependent upon the number of fibers analyzed: as the number of fibers decreases, the calculated deletion abundance increases. Also, most mtDNA deletions appear to occur in a mosaic pattern, varying from cell to cell in size, number and abundance. These data support the hypothesis that mtDNA deletions can focally accumulate to high levels contributing to declines in mass and function of aging skeletal muscle.

Original language	English
Pages (from-to)	91-101
Number of pages	11
Journal	Mechanisms of Ageing and Development
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/0047-6374(95)01611-3
State	Published - Sep 7 1995

Bibliographical note

Funding Information:
We thank the Richard Moss lab for assistance with the fiber bundle dissections, the members of our lab for helpful discussion throughout the course of this work and Drs. Debbie McKenzie, Mike Strand and Greg Cartee for criticism of the manuscript. Supported by NIH grant PO1 AGl1915 (to RW and JMA). This

Funding

We thank the Richard Moss lab for assistance with the fiber bundle dissections, the members of our lab for helpful discussion throughout the course of this work and Drs. Debbie McKenzie, Mike Strand and Greg Cartee for criticism of the manuscript. Supported by NIH grant PO1 AGl1915 (to RW and JMA). This

Funders	Funder number
National Institutes of Health (NIH)	PO1 AGl1915
National Institute on Aging	P01AG011915

Keywords

Deletions
Mitochondrial DNA
Muscle
Quantitation
Rhesus monkeys

ASJC Scopus subject areas

Aging
Developmental Biology

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10.1016/0047-6374(95)01611-3

Cite this

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title = "High levels of mitochondrial DNA deletions in skeletal muscle of old rhesus monkeys",

abstract = "Mitochondrial DNA (mtDNA) deletions increase in abundance with age in many tissues, however, their calculated low levels (usually < 0.1%) in samples from tissue homogenates containing thousands of cells argue against physiologic significance. Through the analysis of defined numbers of cells (skeletal muscle fibers) from rhesus monkeys, we report that the calculated abundance of specific mtDNA deletions is dependent upon the number of fibers analyzed: as the number of fibers decreases, the calculated deletion abundance increases. Also, most mtDNA deletions appear to occur in a mosaic pattern, varying from cell to cell in size, number and abundance. These data support the hypothesis that mtDNA deletions can focally accumulate to high levels contributing to declines in mass and function of aging skeletal muscle.",

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author = "Schwarze, {Steven R.} and Lee, {Connie M.} and Chung, {Susan S.} and Roecker, {Ellen B.} and Richard Weindruch and Aiken, {Judd M.}",

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AU - Schwarze, Steven R.

AU - Lee, Connie M.

AU - Chung, Susan S.

AU - Roecker, Ellen B.

AU - Weindruch, Richard

AU - Aiken, Judd M.

N1 - Funding Information: We thank the Richard Moss lab for assistance with the fiber bundle dissections, the members of our lab for helpful discussion throughout the course of this work and Drs. Debbie McKenzie, Mike Strand and Greg Cartee for criticism of the manuscript. Supported by NIH grant PO1 AGl1915 (to RW and JMA). This

PY - 1995/9/7

Y1 - 1995/9/7

N2 - Mitochondrial DNA (mtDNA) deletions increase in abundance with age in many tissues, however, their calculated low levels (usually < 0.1%) in samples from tissue homogenates containing thousands of cells argue against physiologic significance. Through the analysis of defined numbers of cells (skeletal muscle fibers) from rhesus monkeys, we report that the calculated abundance of specific mtDNA deletions is dependent upon the number of fibers analyzed: as the number of fibers decreases, the calculated deletion abundance increases. Also, most mtDNA deletions appear to occur in a mosaic pattern, varying from cell to cell in size, number and abundance. These data support the hypothesis that mtDNA deletions can focally accumulate to high levels contributing to declines in mass and function of aging skeletal muscle.

AB - Mitochondrial DNA (mtDNA) deletions increase in abundance with age in many tissues, however, their calculated low levels (usually < 0.1%) in samples from tissue homogenates containing thousands of cells argue against physiologic significance. Through the analysis of defined numbers of cells (skeletal muscle fibers) from rhesus monkeys, we report that the calculated abundance of specific mtDNA deletions is dependent upon the number of fibers analyzed: as the number of fibers decreases, the calculated deletion abundance increases. Also, most mtDNA deletions appear to occur in a mosaic pattern, varying from cell to cell in size, number and abundance. These data support the hypothesis that mtDNA deletions can focally accumulate to high levels contributing to declines in mass and function of aging skeletal muscle.

KW - Deletions

KW - Mitochondrial DNA

KW - Muscle

KW - Quantitation

KW - Rhesus monkeys

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High levels of mitochondrial DNA deletions in skeletal muscle of old rhesus monkeys

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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