High-pressure homogenization combined with sulfhydryl blockage by hydrogen peroxide enhance the thermal stability of chicken breast myofibrillar protein aqueous solution

Xing Chen, Youling L. Xiong, Xinglian Xu

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62 Scopus citations

Abstract

This study tested the potential of high-pressure homogenization (HPH, 69 MPa) combined with hydrogen peroxide (H 2 O 2 , at 0, 40, 80, 160 and 320 μmol/g protein) on enhancing the stability of myofibrillar protein (MP, 15 mg/mL) against thermal aggregation (95 °C for 10 min) in aqueous solution. The addition of H 2 O 2 blocked the sulfhydryl (SH) groups, inhibited the formation of disulfide bonds, and suppressed thermal aggregation of MP. HPH facilitated the blockage effect of H 2 O 2 by disrupting the intact myofibril structure and exposing buried –SH groups, and this resultedin stronger inhibition of thermal aggregation therefore improved solubility of MP. More than 75% of heated MP remained soluble after the treatment with HPH and 160 μmol/g H 2 O 2 , while untreated samples formed a gel upon heating. These results proved that HPH combined with H 2 O 2 is an effective strategy to promote heat stability of MP in the development of muscle protein-based beverages.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalFood Chemistry
Volume285
DOIs
StatePublished - Jul 1 2019

Bibliographical note

Funding Information:
This study was supported by the National Key R&D Program of China (No. 2018YFD0401203 ), the USDA National Institute of Food and Agriculture (Hatch Project 1005724), the National Natural Science Foundation of China (No. 31671875 ), an Oversea Study Fellowship from the China Scholarship Council (to Xing Chen), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). Approved for publication as journal article no. 18-07-008 by the Director of the Kentucky Agricultural Experiment Station.

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

  • Heat stability
  • High-pressure homogenization
  • Hydrogen peroxide
  • Myofibrillar protein
  • Protein beverage
  • Sulfhydryl blockage

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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