High-quality combinatorial protein libraries using the binary patterning approach

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Protein combinatorial libraries have become a platform technology for exploring protein sequence space for novel molecules for use in research, synthetic biology, biotechnology, and medicine. To expedite the isolation of proteins with novel/desired functions using screens and selections, high-quality approaches that generate protein libraries rich in folded and soluble structures are desirable for this goal. The binary patterning approach is a protein library design method that incorporates elements of both rational design and combinatorial diversity to specify the arrangement of polar and nonpolar amino acid residues in the context of a desired, folded tertiary structure template. An overview of the considerations necessary to design and construct binary patterned libraries of de novo and natural proteins is presented.

Original languageEnglish
Pages (from-to)117-128
Number of pages12
JournalMethods in Molecular Biology
StatePublished - 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2014.


  • Combinatorial library design
  • Gene library
  • Peptide library
  • Protein design
  • Protein engineering
  • Synthetic biology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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