High-throughput fluorescence correlation spectroscopy enables analysis of surface components of cell-derived vesicles

Xu Fu, Yongwook Song, Abdullah Masud, Kanthi Nuti, Jason E. DeRouchey, Christopher I. Richards

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Extracellular vesicles (EVs) and cell-derived vesicles (CDVs), generated by fragmenting cellular membranes, have both been explored as therapeutic delivery vehicles. Surface proteins on these vesicles are of great importance as they are characteristic to the cell of origin and modulate vesicle interactions with target cells. Here, we introduced a high-throughput fluorescence correlation spectroscopy (ht-FCS) approach capable of characterizing vesicle surface proteins across a large number of samples. We used automated screening and acquisition of FCS data to profile surface proteins of cell-derived vesicles with high fidelity based on changes in diffusion time upon antibody-vesicle interactions. We characterized vesicles generated from 4 cell types using antibodies for known exosome biomarkers. The ht-FCS technique presented here offers the capability to screen EVs or cell-derived vesicles against a library of surface markers or to screen a library of cell-derived vesicles for a specific identifying marker at a high speed.

Original languageEnglish
Pages (from-to)2589-2597
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume412
Issue number11
DOIs
StatePublished - Apr 1 2020

Bibliographical note

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

  • Cell-derived vesicles
  • FCS
  • High-throughput screening
  • Surface protein composition

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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