Histidine tagged protein recovery from tobacco extract by foam fractionation

Czarena Crofcheck, Melanie Loiselle, James Weekley, Indu Maiti, Sitakanta Pattanaik, Paul M Bummer, Michael Jay

Research output: Contribution to journalArticlepeer-review


Tobacco plants have the potential to be used for the production of proteins for pharmaceutical applications. This work describes a novel protein recovery strategy where the protein of interest is "tagged" with a histidine sequence, which forms a complex with cobalt ions and surfactant possessing a chelating functionality, such that the protein is recovered in the foamate of a foam fractionation step. His-gus, a histidine-tagged enzyme, was chosen as a model protein to study the feasibility of this strategy. The His-gus is recovered from spiked prefoamed tobacco extract by foam fractionation in the presence of surfactant and cobalt ions with an enrichment of 1.29 and a recovery of 21.5% in terms of an adjusted activity.

Original languageEnglish
Pages (from-to)680-2
Number of pages3
JournalBiotechnology Progress
Issue number2
StatePublished - 2003


  • Chemical Fractionation/methods
  • Enzyme Activation
  • Feasibility Studies
  • Glucuronidase/biosynthesis
  • Histidine/chemistry
  • Plant Extracts/chemistry
  • Plant Leaves/chemistry
  • Protein Binding
  • Recombinant Fusion Proteins/biosynthesis
  • Surface-Active Agents/chemistry
  • Tobacco/chemistry


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