TY - JOUR
T1 - Histologically, immunohistochemically, ultrastructurally, and molecularly confirmed neosporosis abortion in an aborted equine fetus
AU - Anderson, Joseph A.
AU - Alves, Derron A.
AU - Cerqueira-Cézar, Camila K.
AU - da Silva, Andressa F.
AU - Murata, Fernando H.A.
AU - Norris, Jamie K.
AU - Howe, Daniel K.
AU - Dubey, Jitender P.
N1 - Publisher Copyright:
© 2019
PY - 2019/6
Y1 - 2019/6
N2 - Neosporosis is a common cause of abortion in cattle worldwide but is rare in horses. Here, the first case of histologically, ultrastructurally, immunohistochemically, and molecularly confirmed equine abortion caused by neosporosis is reported. Samples of lung, heart, liver, skeletal muscle, tongue, brain, and the placenta from a female fetus aborted at 280 days of gestation were fixed in formalin and submitted for diagnosis. Histologically, there was disseminated neosporosis with severe lesions in lungs, liver and the heart. Protozoal tachyzoites in all tissues reacted with polyclonal anti-Neospora caninum rabbit antibodies. Transmission electron microscopic observation on lung tissue revealed tachyzoites consistent with Neospora, including many rhoptries. Polymerase-chain reaction (PCR)using primers designed to amplify the rRNA gene internal transcribed spacer 1 (ITS1)of the Sarcocystidae was performed on DNA extracted from fetal tissues. Comparison of the ITS1 amplified from the foal tissue to sequences available in GenBank revealed 100% sequence identity to the ITS1 from three isolates of Neospora hughesi.
AB - Neosporosis is a common cause of abortion in cattle worldwide but is rare in horses. Here, the first case of histologically, ultrastructurally, immunohistochemically, and molecularly confirmed equine abortion caused by neosporosis is reported. Samples of lung, heart, liver, skeletal muscle, tongue, brain, and the placenta from a female fetus aborted at 280 days of gestation were fixed in formalin and submitted for diagnosis. Histologically, there was disseminated neosporosis with severe lesions in lungs, liver and the heart. Protozoal tachyzoites in all tissues reacted with polyclonal anti-Neospora caninum rabbit antibodies. Transmission electron microscopic observation on lung tissue revealed tachyzoites consistent with Neospora, including many rhoptries. Polymerase-chain reaction (PCR)using primers designed to amplify the rRNA gene internal transcribed spacer 1 (ITS1)of the Sarcocystidae was performed on DNA extracted from fetal tissues. Comparison of the ITS1 amplified from the foal tissue to sequences available in GenBank revealed 100% sequence identity to the ITS1 from three isolates of Neospora hughesi.
KW - Diagnosis
KW - Horse
KW - Molecular
KW - Neospora, abortion
KW - Tachyzoites
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U2 - 10.1016/j.vetpar.2019.04.009
DO - 10.1016/j.vetpar.2019.04.009
M3 - Article
C2 - 31213237
AN - SCOPUS:85065741186
SN - 0304-4017
VL - 270
SP - 20
EP - 24
JO - Veterinary Parasitology
JF - Veterinary Parasitology
ER -