Histologically, immunohistochemically, ultrastructurally, and molecularly confirmed neosporosis abortion in an aborted equine fetus

Joseph A. Anderson, Derron A. Alves, Camila K. Cerqueira-Cézar, Andressa F. da Silva, Fernando H.A. Murata, Jamie K. Norris, Daniel K. Howe, Jitender P. Dubey

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Neosporosis is a common cause of abortion in cattle worldwide but is rare in horses. Here, the first case of histologically, ultrastructurally, immunohistochemically, and molecularly confirmed equine abortion caused by neosporosis is reported. Samples of lung, heart, liver, skeletal muscle, tongue, brain, and the placenta from a female fetus aborted at 280 days of gestation were fixed in formalin and submitted for diagnosis. Histologically, there was disseminated neosporosis with severe lesions in lungs, liver and the heart. Protozoal tachyzoites in all tissues reacted with polyclonal anti-Neospora caninum rabbit antibodies. Transmission electron microscopic observation on lung tissue revealed tachyzoites consistent with Neospora, including many rhoptries. Polymerase-chain reaction (PCR)using primers designed to amplify the rRNA gene internal transcribed spacer 1 (ITS1)of the Sarcocystidae was performed on DNA extracted from fetal tissues. Comparison of the ITS1 amplified from the foal tissue to sequences available in GenBank revealed 100% sequence identity to the ITS1 from three isolates of Neospora hughesi.

Original languageEnglish
Pages (from-to)20-24
Number of pages5
JournalVeterinary Parasitology
StatePublished - Jun 2019

Bibliographical note

Funding Information:
This research was supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-SC0014664. All opinions expressed in this paper are the author's and do not necessarily reflect the policies and views of USDA, ARS, DOE, or ORAU/ORISE.

Publisher Copyright:
© 2019


  • Diagnosis
  • Horse
  • Molecular
  • Neospora, abortion
  • Tachyzoites

ASJC Scopus subject areas

  • Parasitology
  • Veterinary (all)


Dive into the research topics of 'Histologically, immunohistochemically, ultrastructurally, and molecularly confirmed neosporosis abortion in an aborted equine fetus'. Together they form a unique fingerprint.

Cite this