Homology throughout the multiple 32-kilobase circular plasmids present in lyme disease spirochetes

Sherwood Casjens, René Van Vugt, Kit Tilly, Patricia A. Rosa, Brian Stevenson

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

We have characterized seven different 32-kb circular plasmids carried by Borrelia burgdorferi isolate B31. Restriction endonuclease recognition site mapping and partial sequencing of these plasmids indicated that all seven are probably closely related to each other throughout their lengths and have substantial relationships to cp8.3, an 8.3-kb circular plasmid of B. burgdorferi sensu lato isolate Ip21. With the addition of the seven 32-kb plasmids, this bacterial strain is known to carry at least 10 linear and 9 circular plasmids. Variant cultures of B. burgdorferi, B31 lacking nne or more of the 32-kb circular plasmids are viable and, at least in some cases, infectious. We have examined a number of different natural isolates of Lyme disease borreliae and found that all of the B. burgdorferi sensu stricto isolates and most of the B. burgdorferi sensu lato isolates tested appear to carry multiple 32-kh circular plasmids related to those of B. burgdorferi B31. The ubiquity of these plasmids suggests that they may be important in the natural life cycle of these organisms. They may be highly conjugative plasmids or prophage genomes, which could prove to he useful in genetically manipulating B. burgdorferi.

Original languageEnglish
Pages (from-to)217-227
Number of pages11
JournalJournal of Bacteriology
Volume179
Issue number1
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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