TY - JOUR
T1 - Homology throughout the multiple 32-kilobase circular plasmids present in lyme disease spirochetes
AU - Casjens, Sherwood
AU - Van Vugt, René
AU - Tilly, Kit
AU - Rosa, Patricia A.
AU - Stevenson, Brian
PY - 1997
Y1 - 1997
N2 - We have characterized seven different 32-kb circular plasmids carried by Borrelia burgdorferi isolate B31. Restriction endonuclease recognition site mapping and partial sequencing of these plasmids indicated that all seven are probably closely related to each other throughout their lengths and have substantial relationships to cp8.3, an 8.3-kb circular plasmid of B. burgdorferi sensu lato isolate Ip21. With the addition of the seven 32-kb plasmids, this bacterial strain is known to carry at least 10 linear and 9 circular plasmids. Variant cultures of B. burgdorferi, B31 lacking nne or more of the 32-kb circular plasmids are viable and, at least in some cases, infectious. We have examined a number of different natural isolates of Lyme disease borreliae and found that all of the B. burgdorferi sensu stricto isolates and most of the B. burgdorferi sensu lato isolates tested appear to carry multiple 32-kh circular plasmids related to those of B. burgdorferi B31. The ubiquity of these plasmids suggests that they may be important in the natural life cycle of these organisms. They may be highly conjugative plasmids or prophage genomes, which could prove to he useful in genetically manipulating B. burgdorferi.
AB - We have characterized seven different 32-kb circular plasmids carried by Borrelia burgdorferi isolate B31. Restriction endonuclease recognition site mapping and partial sequencing of these plasmids indicated that all seven are probably closely related to each other throughout their lengths and have substantial relationships to cp8.3, an 8.3-kb circular plasmid of B. burgdorferi sensu lato isolate Ip21. With the addition of the seven 32-kb plasmids, this bacterial strain is known to carry at least 10 linear and 9 circular plasmids. Variant cultures of B. burgdorferi, B31 lacking nne or more of the 32-kb circular plasmids are viable and, at least in some cases, infectious. We have examined a number of different natural isolates of Lyme disease borreliae and found that all of the B. burgdorferi sensu stricto isolates and most of the B. burgdorferi sensu lato isolates tested appear to carry multiple 32-kh circular plasmids related to those of B. burgdorferi B31. The ubiquity of these plasmids suggests that they may be important in the natural life cycle of these organisms. They may be highly conjugative plasmids or prophage genomes, which could prove to he useful in genetically manipulating B. burgdorferi.
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U2 - 10.1128/jb.179.1.217-227.1997
DO - 10.1128/jb.179.1.217-227.1997
M3 - Article
C2 - 8982001
AN - SCOPUS:0031012029
SN - 0021-9193
VL - 179
SP - 217
EP - 227
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 1
ER -