TY - JOUR
T1 - Horse Y chromosome assembly displays unique evolutionary features and putative stallion fertility genes
AU - Janečka, Jan E.
AU - Davis, Brian W.
AU - Ghosh, Sharmila
AU - Paria, Nandina
AU - Das, Pranab J.
AU - Orlando, Ludovic
AU - Schubert, Mikkel
AU - Nielsen, Martin K.
AU - Stout, Tom A.E.
AU - Brashear, Wesley
AU - Li, Gang
AU - Johnson, Charles D.
AU - Metz, Richard P.
AU - Zadjali, Al Muatasim Al
AU - Love, Charles C.
AU - Varner, Dickson D.
AU - Bellott, Daniel W.
AU - Murphy, William J.
AU - Chowdhary, Bhanu P.
AU - Raudsepp, Terje
N1 - Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Dynamic evolutionary processes and complex structure make the Y chromosome among the most diverse and least understood regions in mammalian genomes. Here, we present an annotated assembly of the male specific region of the horse Y chromosome (eMSY), representing the first comprehensive Y assembly in odd-toed ungulates. The eMSY comprises single-copy, equine specific multi-copy, PAR transposed, and novel ampliconic sequence classes. The eMSY gene density approaches that of autosomes with the highest number of retained X–Y gametologs recorded in eutherians, in addition to novel Y-born and transposed genes. Horse, donkey and mule testis RNAseq reveals several candidate genes for stallion fertility. A novel testis-expressed XY ampliconic sequence class, ETSTY7, is shared with the parasite Parascaris genome, providing evidence for eukaryotic horizontal transfer and inter-chromosomal mobility. Our study highlights the dynamic nature of the Y and provides a reference sequence for improved understanding of equine male development and fertility.
AB - Dynamic evolutionary processes and complex structure make the Y chromosome among the most diverse and least understood regions in mammalian genomes. Here, we present an annotated assembly of the male specific region of the horse Y chromosome (eMSY), representing the first comprehensive Y assembly in odd-toed ungulates. The eMSY comprises single-copy, equine specific multi-copy, PAR transposed, and novel ampliconic sequence classes. The eMSY gene density approaches that of autosomes with the highest number of retained X–Y gametologs recorded in eutherians, in addition to novel Y-born and transposed genes. Horse, donkey and mule testis RNAseq reveals several candidate genes for stallion fertility. A novel testis-expressed XY ampliconic sequence class, ETSTY7, is shared with the parasite Parascaris genome, providing evidence for eukaryotic horizontal transfer and inter-chromosomal mobility. Our study highlights the dynamic nature of the Y and provides a reference sequence for improved understanding of equine male development and fertility.
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U2 - 10.1038/s41467-018-05290-6
DO - 10.1038/s41467-018-05290-6
M3 - Article
C2 - 30054462
AN - SCOPUS:85050817035
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 2945
ER -