Strain-specific factors contribute in significant but undefined ways to the variable incidence of herpes simplex virus (HSV) recrudescence. Studies that investigate these strain-specific factors are needed. Here, we used qPCR, in vitro assays, and genomic sequencing to identify important relationships between in vitro and clinical phenotypes of unique HSV-1 clinical isolates. Nine HSV-1 isolates from individuals displaying varying reactivation patterns were studied. Isolates associated with frequent recurrent herpes labialis (RHL) (1) displayed higher rates of viral shedding in the oral cavity than those associated with rare RHL and (2) tended to replicate more efficiently at 33 °C than 39 °C. HSV-1 isolates also displayed a more stable phenotype during propagation in U2OS cells than in Vero cells. Draft genome sequences of four isolates and one variant spanning 95.6 to 97.2 % of the genome were achieved, and whole-genome alignment demonstrated that the majority of these isolates clustered with known North American/European isolates. These findings revealed procedures that could help identify unique genotypes and phenotypes associated with HSV-1 isolates, which can be important for determining viral factors critical for regulating HSV-1 reactivation.
|Number of pages||15|
|Journal||Journal of NeuroVirology|
|State||Published - Apr 1 2017|
Bibliographical noteFunding Information:
This investigation was supported by NIH COBRE grant 2P20RR020145 and by NIH grant U54AI084844.
© 2016, Journal of NeuroVirology, Inc.
- Genetic variation
- Herpes virus 1
- Human/isolation and purification
- Virus replication
ASJC Scopus subject areas
- Clinical Neurology
- Cellular and Molecular Neuroscience