Human adenovirus replication and persistence in hypertrophic adenoids and palatine tonsils in children

José Luiz Proenca-Modena, Ricardo de Souza Cardoso, Miriã Ferreira Criado, Guilherme Paier Milanez, William Marciel de Souza, Pierina Lorencini Parise, Jéssica Wildgrube Bertol, Bruna Lais Santos de Jesus, Mirela Cristina Moreira Prates, Maria Lúcia Silva, Guilherme Pietrucci Buzatto, Ricardo Cassiano Demarco, Fabiana Cardoso Pereira Valera, Edwin Tamashiro, Wilma Terezinha Anselmo-Lima, Eurico Arruda

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


The role of human adenovirus (HAdV) infection in different acute diseases, such as febrile exudative tonsillitis, conjunctivitis, and pharyngoconjunctival fever is well established. However, the relationships, if any, of HAdV persistence and reactivation in the development of the chronic adenotonsillar disease is not fully understood. The present paper reports a 3-year cross-sectional hospital-based study aimed at detecting and quantifying HAdV DNA and mRNA of the HAdV hexon gene in adenoid and palatine tonsil tissues and nasopharyngeal secretions (NPS) from patients with adenotonsillar hypertrophy or recurrent adenotonsillitis. HAdV C, B, and E were detectable in nearly 50% of the patients, with no association with the severity of airway obstruction, nor with the presence of recurrent tonsillitis, sleep apnea or otitis media with effusion (OME). Despite the higher rates of respiratory viral coinfections in patients with HAdV, the presence of other viruses, including DNA and RNA viruses, had no association with HAdV replication or shedding in secretions. Higher HAdV loads in adenoids showed a significant positive correlation with the presence of sleep apnea and the absence of OME. Although this study indicates that a significant proportion (~85%) of individuals with chronic adenotonsillar diseases have persistent nonproductive HAdV infection, including those by HAdV C, B, and E, epithelial and subepithelial cells in tonsils seem to be critical for HAdV C production and shedding in NPS in some patients, since viral antigen was detected in these regions by immunohistochemistry in four patients, all of which were also positive for HAdV mRNA detection.

Original languageEnglish
Pages (from-to)1250-1262
Number of pages13
JournalJournal of Medical Virology
Issue number7
StatePublished - Jul 2019

Bibliographical note

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.


  • chronic adenotonsillar disease
  • hexon mRNA
  • human adenovirus (HAdV)

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology


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