TY - JOUR
T1 - Human Neural Stem Cell Extracellular Vesicles Improve Tissue and Functional Recovery in the Murine Thromboembolic Stroke Model
AU - Webb, Robin L.
AU - Kaiser, Erin E.
AU - Scoville, Shelley L.
AU - Thompson, Tyler A.
AU - Fatima, Sumbul
AU - Pandya, Chirayukumar
AU - Sriram, Karishma
AU - Swetenburg, Raymond L.
AU - Vaibhav, Kumar
AU - Arbab, Ali S.
AU - Baban, Babak
AU - Dhandapani, Krishnan M.
AU - Hess, David C.
AU - Hoda, M. N.
AU - Stice, Steven L.
N1 - Publisher Copyright:
© 2017, The Author(s).
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Over 700 drugs have failed in stroke clinical trials, an unprecedented rate thought to be attributed in part to limited and isolated testing often solely in “young” rodent models and focusing on a single secondary injury mechanism. Here, extracellular vesicles (EVs), nanometer-sized cell signaling particles, were tested in a mouse thromboembolic (TE) stroke model. Neural stem cell (NSC) and mesenchymal stem cell (MSC) EVs derived from the same pluripotent stem cell (PSC) line were evaluated for changes in infarct volume as well as sensorimotor function. NSC EVs improved cellular, tissue, and functional outcomes in middle-aged rodents, whereas MSC EVs were less effective. Acute differences in lesion volume following NSC EV treatment were corroborated by MRI in 18-month-old aged rodents. NSC EV treatment has a positive effect on motor function in the aged rodent as indicated by beam walk, instances of foot faults, and strength evaluated by hanging wire test. Increased time with a novel object also indicated that NSC EVs improved episodic memory formation in the rodent. The therapeutic effect of NSC EVs appears to be mediated by altering the systemic immune response. These data strongly support further preclinical development of a NSC EV-based stroke therapy and warrant their testing in combination with FDA-approved stroke therapies.
AB - Over 700 drugs have failed in stroke clinical trials, an unprecedented rate thought to be attributed in part to limited and isolated testing often solely in “young” rodent models and focusing on a single secondary injury mechanism. Here, extracellular vesicles (EVs), nanometer-sized cell signaling particles, were tested in a mouse thromboembolic (TE) stroke model. Neural stem cell (NSC) and mesenchymal stem cell (MSC) EVs derived from the same pluripotent stem cell (PSC) line were evaluated for changes in infarct volume as well as sensorimotor function. NSC EVs improved cellular, tissue, and functional outcomes in middle-aged rodents, whereas MSC EVs were less effective. Acute differences in lesion volume following NSC EV treatment were corroborated by MRI in 18-month-old aged rodents. NSC EV treatment has a positive effect on motor function in the aged rodent as indicated by beam walk, instances of foot faults, and strength evaluated by hanging wire test. Increased time with a novel object also indicated that NSC EVs improved episodic memory formation in the rodent. The therapeutic effect of NSC EVs appears to be mediated by altering the systemic immune response. These data strongly support further preclinical development of a NSC EV-based stroke therapy and warrant their testing in combination with FDA-approved stroke therapies.
KW - Neural stem cell extracellular vesicles
KW - Preclinical stroke model
KW - Thromboembolic stroke
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U2 - 10.1007/s12975-017-0599-2
DO - 10.1007/s12975-017-0599-2
M3 - Article
C2 - 29285679
AN - SCOPUS:85039547348
SN - 1868-4483
VL - 9
SP - 530
EP - 539
JO - Translational Stroke Research
JF - Translational Stroke Research
IS - 5
ER -