Htra2-beta is a human homologue of Drosophila transformer-2 and a member of the SR-like protein family. Here we report the isolation and characterization of the complete htra2-beta gene (HGMW-approved symbol SFRS10). The gene spans 21,232 bp and is composed of 10 exons and 9 introns. Radiation hybrid mapping localized the gene to chromosome 3q. The region upstream of the transcription initiation codon contains an Alu element and several potential transcription factor binding sites. RT-PCR and comparison with EST clones revealed five different RNA isoforms generated by alternative splicing. These isoforms encode three diverging open reading frames, and two of these, htra2-beta3 and htra2-beta4, lack the first SR domain. Htra2-beta3 is developmentally regulated and expressed predominantly in brain, liver testis, and weakly in kidney. Furthermore, the domain structure of htra2- beta3 resembles a variant found in the Drosophila male germline, indicating a remarkable conservation of alternative transformer-2 variants. Finally, we show that htra2-beta3 is expressed in the nucleus and interacts with a subset of SR proteins in a yeast two-hybrid system and in vivo.
|Number of pages||12|
|State||Published - Oct 15 1998|
Bibliographical noteFunding Information:
This work was supported by the Max-Planck Society, the Human Science Frontier Program (RG 562/96 to S.S.), and the Swiss National Science Foundation (O.N.). We thank Prof. A. Ullrich for his support, Toni Weinschenck for technical assistance, Jim Chalcroft for artwork, and Petra Kioschis (German Human Genome Project, Heidelberg) for performing the genomic screen.
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