The human umbilical cord blood (HUCB) mononuclear cell (MNC) fraction is a mixed population of cells that induces functional repair in rodent models of stroke when injected intravenously (i. v.). The transplanted cells are found in the infarcted hemisphere and the spleen. The goal of this project was to determine the nature of the interaction between the HUCB MNCs and splenic immune cells. Male Sprague Dawley rats underwent permanent middle cerebral artery occlusion (MCAO) and received i. v. injection of either vehicle (MCAO only), HUCB MNCs, or MNCs depleted of CD14+ monocytes, CD133+ stem cells, or CD19+ B cells 48 h poststroke. At 72 h post-MCAO, the animals were euthanized, and the spleens and blood MNCs were harvested for flow cytometry and mitogen proliferation assays. All HUCB cell preparations decreased the percentage of T cells in the spleen and monocytes in the blood (p < 0. 05). MNCs depleted of CD14+ and CD19+ decreased the percentage of macrophage (p < 0. 001), while CD133-depleted MNCs increased the percentage of macrophage in the spleen (p < 0. 001); MNC did not alter the macrophage population from the level observed after MCAO. Only HUCB MNC significantly decreased concanavalin A-induced T cell stimulation (p < 0. 05). These results suggest that the effects of HUCB MNC in the spleen are not due to a single HUCB population, but the interaction of all the subpopulations together.
|Number of pages||9|
|Journal||Translational Stroke Research|
|State||Published - Dec 2012|
Bibliographical noteFunding Information:
Acknowledgments This research was supported in part by the Florida Department of Health, James and Esther King Biomedical Research Program (07 KB-07), and the National Institute of Neurological Diseases and Stroke (RO1NS52839 to AEW). AEWand PRS are inventors on cord blood related patents. AEW is a consultant to Saneron CCEL Therapeutics, Inc. and PRS is co-founder of Saneron CCEL Therapeutics, Inc.
- B cells
- Mononuclear cells
- T cells
ASJC Scopus subject areas
- Neuroscience (all)
- Clinical Neurology
- Cardiology and Cardiovascular Medicine