Human Umbilical Cord Blood Cells Alter Blood and Spleen Cell Populations After Stroke

J. E. Golden; M. Shahaduzzaman; A. Wabnitz; S. Green; T. A. Womble; P. R. Sanberg; K. R. Pennypacker; A. E. Willing

doi:10.1007/s12975-012-0208-3

Human Umbilical Cord Blood Cells Alter Blood and Spleen Cell Populations After Stroke

J. E. Golden, M. Shahaduzzaman, A. Wabnitz, S. Green, T. A. Womble, P. R. Sanberg, K. R. Pennypacker, A. E. Willing

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The human umbilical cord blood (HUCB) mononuclear cell (MNC) fraction is a mixed population of cells that induces functional repair in rodent models of stroke when injected intravenously (i. v.). The transplanted cells are found in the infarcted hemisphere and the spleen. The goal of this project was to determine the nature of the interaction between the HUCB MNCs and splenic immune cells. Male Sprague Dawley rats underwent permanent middle cerebral artery occlusion (MCAO) and received i. v. injection of either vehicle (MCAO only), HUCB MNCs, or MNCs depleted of CD14+ monocytes, CD133+ stem cells, or CD19+ B cells 48 h poststroke. At 72 h post-MCAO, the animals were euthanized, and the spleens and blood MNCs were harvested for flow cytometry and mitogen proliferation assays. All HUCB cell preparations decreased the percentage of T cells in the spleen and monocytes in the blood (p < 0. 05). MNCs depleted of CD14+ and CD19+ decreased the percentage of macrophage (p < 0. 001), while CD133-depleted MNCs increased the percentage of macrophage in the spleen (p < 0. 001); MNC did not alter the macrophage population from the level observed after MCAO. Only HUCB MNC significantly decreased concanavalin A-induced T cell stimulation (p < 0. 05). These results suggest that the effects of HUCB MNC in the spleen are not due to a single HUCB population, but the interaction of all the subpopulations together.

Original language	English
Pages (from-to)	491-499
Number of pages	9
Journal	Translational Stroke Research
Volume	3
Issue number	4
DOIs	https://doi.org/10.1007/s12975-012-0208-3
State	Published - Dec 2012

Bibliographical note

Funding Information:
Acknowledgments This research was supported in part by the Florida Department of Health, James and Esther King Biomedical Research Program (07 KB-07), and the National Institute of Neurological Diseases and Stroke (RO1NS52839 to AEW). AEWand PRS are inventors on cord blood related patents. AEW is a consultant to Saneron CCEL Therapeutics, Inc. and PRS is co-founder of Saneron CCEL Therapeutics, Inc.

Keywords

B cells
Macrophage
Monocytes
Mononuclear cells
Stroke
T cells

ASJC Scopus subject areas

General Neuroscience
Clinical Neurology
Cardiology and Cardiovascular Medicine

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10.1007/s12975-012-0208-3

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title = "Human Umbilical Cord Blood Cells Alter Blood and Spleen Cell Populations After Stroke",

abstract = "The human umbilical cord blood (HUCB) mononuclear cell (MNC) fraction is a mixed population of cells that induces functional repair in rodent models of stroke when injected intravenously (i. v.). The transplanted cells are found in the infarcted hemisphere and the spleen. The goal of this project was to determine the nature of the interaction between the HUCB MNCs and splenic immune cells. Male Sprague Dawley rats underwent permanent middle cerebral artery occlusion (MCAO) and received i. v. injection of either vehicle (MCAO only), HUCB MNCs, or MNCs depleted of CD14+ monocytes, CD133+ stem cells, or CD19+ B cells 48 h poststroke. At 72 h post-MCAO, the animals were euthanized, and the spleens and blood MNCs were harvested for flow cytometry and mitogen proliferation assays. All HUCB cell preparations decreased the percentage of T cells in the spleen and monocytes in the blood (p < 0. 05). MNCs depleted of CD14+ and CD19+ decreased the percentage of macrophage (p < 0. 001), while CD133-depleted MNCs increased the percentage of macrophage in the spleen (p < 0. 001); MNC did not alter the macrophage population from the level observed after MCAO. Only HUCB MNC significantly decreased concanavalin A-induced T cell stimulation (p < 0. 05). These results suggest that the effects of HUCB MNC in the spleen are not due to a single HUCB population, but the interaction of all the subpopulations together.",

keywords = "B cells, Macrophage, Monocytes, Mononuclear cells, Stroke, T cells",

author = "Golden, {J. E.} and M. Shahaduzzaman and A. Wabnitz and S. Green and Womble, {T. A.} and Sanberg, {P. R.} and Pennypacker, {K. R.} and Willing, {A. E.}",

note = "Funding Information: Acknowledgments This research was supported in part by the Florida Department of Health, James and Esther King Biomedical Research Program (07 KB-07), and the National Institute of Neurological Diseases and Stroke (RO1NS52839 to AEW). AEWand PRS are inventors on cord blood related patents. AEW is a consultant to Saneron CCEL Therapeutics, Inc. and PRS is co-founder of Saneron CCEL Therapeutics, Inc.",

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AU - Womble, T. A.

AU - Sanberg, P. R.

AU - Pennypacker, K. R.

AU - Willing, A. E.

N1 - Funding Information: Acknowledgments This research was supported in part by the Florida Department of Health, James and Esther King Biomedical Research Program (07 KB-07), and the National Institute of Neurological Diseases and Stroke (RO1NS52839 to AEW). AEWand PRS are inventors on cord blood related patents. AEW is a consultant to Saneron CCEL Therapeutics, Inc. and PRS is co-founder of Saneron CCEL Therapeutics, Inc.

PY - 2012/12

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N2 - The human umbilical cord blood (HUCB) mononuclear cell (MNC) fraction is a mixed population of cells that induces functional repair in rodent models of stroke when injected intravenously (i. v.). The transplanted cells are found in the infarcted hemisphere and the spleen. The goal of this project was to determine the nature of the interaction between the HUCB MNCs and splenic immune cells. Male Sprague Dawley rats underwent permanent middle cerebral artery occlusion (MCAO) and received i. v. injection of either vehicle (MCAO only), HUCB MNCs, or MNCs depleted of CD14+ monocytes, CD133+ stem cells, or CD19+ B cells 48 h poststroke. At 72 h post-MCAO, the animals were euthanized, and the spleens and blood MNCs were harvested for flow cytometry and mitogen proliferation assays. All HUCB cell preparations decreased the percentage of T cells in the spleen and monocytes in the blood (p < 0. 05). MNCs depleted of CD14+ and CD19+ decreased the percentage of macrophage (p < 0. 001), while CD133-depleted MNCs increased the percentage of macrophage in the spleen (p < 0. 001); MNC did not alter the macrophage population from the level observed after MCAO. Only HUCB MNC significantly decreased concanavalin A-induced T cell stimulation (p < 0. 05). These results suggest that the effects of HUCB MNC in the spleen are not due to a single HUCB population, but the interaction of all the subpopulations together.

AB - The human umbilical cord blood (HUCB) mononuclear cell (MNC) fraction is a mixed population of cells that induces functional repair in rodent models of stroke when injected intravenously (i. v.). The transplanted cells are found in the infarcted hemisphere and the spleen. The goal of this project was to determine the nature of the interaction between the HUCB MNCs and splenic immune cells. Male Sprague Dawley rats underwent permanent middle cerebral artery occlusion (MCAO) and received i. v. injection of either vehicle (MCAO only), HUCB MNCs, or MNCs depleted of CD14+ monocytes, CD133+ stem cells, or CD19+ B cells 48 h poststroke. At 72 h post-MCAO, the animals were euthanized, and the spleens and blood MNCs were harvested for flow cytometry and mitogen proliferation assays. All HUCB cell preparations decreased the percentage of T cells in the spleen and monocytes in the blood (p < 0. 05). MNCs depleted of CD14+ and CD19+ decreased the percentage of macrophage (p < 0. 001), while CD133-depleted MNCs increased the percentage of macrophage in the spleen (p < 0. 001); MNC did not alter the macrophage population from the level observed after MCAO. Only HUCB MNC significantly decreased concanavalin A-induced T cell stimulation (p < 0. 05). These results suggest that the effects of HUCB MNC in the spleen are not due to a single HUCB population, but the interaction of all the subpopulations together.

KW - B cells

KW - Macrophage

KW - Monocytes

KW - Mononuclear cells

KW - Stroke

KW - T cells

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SP - 491

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JO - Translational Stroke Research

JF - Translational Stroke Research

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ER -

Human Umbilical Cord Blood Cells Alter Blood and Spleen Cell Populations After Stroke

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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