HUWE1 Is a molecular link controlling RAF-1 activity supported by the Shoc2 scaffold

Eun Ryoung Jang, Ping Shi, Jamal Bryant, Jing Chen, Vikas Dukhande, Matthew S. Gentry, Hye In Jang, Myoungkun Jeoung, Emilia Galperin

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Scaffold proteins play a critical role in controlling the activity of the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway. Shoc2 is a leucine-rich repeat scaffold protein that acts as a positive modulator of ERK1/2 signaling. However, the precise mechanism by which Shoc2 modulates the activity of the ERK1/2 pathway is unclear. Here we report the identification of the E3 ubiquitin ligase HUWE1 as a binding partner and regulator of Shoc2 function. HUWE1 mediates ubiquitination and, consequently, the levels of Shoc2. Additionally, we show that both Shoc2 and HUWE1 are necessary to control the levels and ubiquitination of the Shoc2 signaling partner, RAF-1. Depletion of HUWE1 abolishes RAF-1 ubiquitination, with corresponding changes in ERK1/2 pathway activity occurring. Our results indicate that the HUWE1-mediated ubiquitination of Shoc2 is the switch that regulates the transition from an active to an inactive state of the RAF-1 kinase. Taken together, our results demonstrate that HUWE1 is a novel player involved in regulating ERK1/2 signal transmission through the Shoc2 scaffold complex.

Original languageEnglish
Pages (from-to)3579-3593
Number of pages15
JournalMolecular and Cellular Biology
Volume34
Issue number19
DOIs
StatePublished - 2014

Bibliographical note

Publisher Copyright:
© 2014, American Society for Microbiology.

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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