Hybrid polymer/porous silicon nanofibers for loading and sustained release of synthetic DNA-based responsive devices

Jonathan M. Zuidema; Alessandro Bertucci; Jinyoung Kang; Michael J. Sailor; Francesco Ricci

doi:10.1039/c9nr08474f

Hybrid polymer/porous silicon nanofibers for loading and sustained release of synthetic DNA-based responsive devices

Jonathan M. Zuidema
, Alessandro Bertucci
, Jinyoung Kang
, Michael J. Sailor
, Francesco Ricci

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Synthetic DNA-based oligonucleotides are loaded into porous silicon nanoparticles (pSiNPs) and incorporated into nanofibers of poly(lactide-co-glycolide) (PLGA), poly-l-lactic acid (PLA), or polycaprolactone (PCL). The resulting hybrid nanofibers are characterized for their ability to release the functional oligonucleotide payload under physiologic conditions. Under temperature and pH conditions mimicking physiological values, the PLGA-based nanofibers release >80% of their DNA cargo within 5 days, whereas the PLA and PCL-based fibers require 15 days to release >80% of their cargo. The quantity of DNA released scales with the quantity of DNA-loaded pSiNPs embedded in the nanofibers; mass loadings of between 2.4 and 9.1% (based on mass of DNA-pSiNP construct relative to mass of polymer composite) are investigated. When a responsive DNA-based nanodevice (i.e. molecular beacon) is used as a payload, it retains its functionality during the release period, independent of the polymer used for the formation of the nanofibers.

Original language	English
Pages (from-to)	2333-2339
Number of pages	7
Journal	Nanoscale
Volume	12
Issue number	4
DOIs	https://doi.org/10.1039/c9nr08474f
State	Published - Jan 28 2020

Bibliographical note

Publisher Copyright:
© 2020 The Royal Society of Chemistry.

Funding

This study was supported by the National Science Foundation under Grant No. CBET-1603177 (MJS). Transmission electron micrographs were taken in the Cellular and Molecular Medicine Electron microscopy core facility, which is supported in part by National Institutes of Health Award number S10OD023527. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no 704120 (“MIRNANO”) and by PRIN 2015 (Grant No. 2015TWP83Z) (F. R.). J. K. acknowledges financial support from the UCSD Frontiers of Innovation Scholars Program (FISP) fellowship. A. B. is a Global Marie Sklodowska-Curie Fellow.

Funders	Funder number
University of California San Diego Health
National Institutes of Health (NIH)	S10OD023527
Horizon 2020 Framework Programme	778133, 704120
PRIN	2015TWP83Z
National Science Foundation Arctic Social Science Program	CBET-1603177

ASJC Scopus subject areas

General Materials Science

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10.1039/c9nr08474f

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title = "Hybrid polymer/porous silicon nanofibers for loading and sustained release of synthetic DNA-based responsive devices",

abstract = "Synthetic DNA-based oligonucleotides are loaded into porous silicon nanoparticles (pSiNPs) and incorporated into nanofibers of poly(lactide-co-glycolide) (PLGA), poly-l-lactic acid (PLA), or polycaprolactone (PCL). The resulting hybrid nanofibers are characterized for their ability to release the functional oligonucleotide payload under physiologic conditions. Under temperature and pH conditions mimicking physiological values, the PLGA-based nanofibers release >80\% of their DNA cargo within 5 days, whereas the PLA and PCL-based fibers require 15 days to release >80\% of their cargo. The quantity of DNA released scales with the quantity of DNA-loaded pSiNPs embedded in the nanofibers; mass loadings of between 2.4 and 9.1\% (based on mass of DNA-pSiNP construct relative to mass of polymer composite) are investigated. When a responsive DNA-based nanodevice (i.e. molecular beacon) is used as a payload, it retains its functionality during the release period, independent of the polymer used for the formation of the nanofibers.",

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AU - Zuidema, Jonathan M.

AU - Bertucci, Alessandro

AU - Kang, Jinyoung

AU - Sailor, Michael J.

AU - Ricci, Francesco

PY - 2020/1/28

Y1 - 2020/1/28

N2 - Synthetic DNA-based oligonucleotides are loaded into porous silicon nanoparticles (pSiNPs) and incorporated into nanofibers of poly(lactide-co-glycolide) (PLGA), poly-l-lactic acid (PLA), or polycaprolactone (PCL). The resulting hybrid nanofibers are characterized for their ability to release the functional oligonucleotide payload under physiologic conditions. Under temperature and pH conditions mimicking physiological values, the PLGA-based nanofibers release >80% of their DNA cargo within 5 days, whereas the PLA and PCL-based fibers require 15 days to release >80% of their cargo. The quantity of DNA released scales with the quantity of DNA-loaded pSiNPs embedded in the nanofibers; mass loadings of between 2.4 and 9.1% (based on mass of DNA-pSiNP construct relative to mass of polymer composite) are investigated. When a responsive DNA-based nanodevice (i.e. molecular beacon) is used as a payload, it retains its functionality during the release period, independent of the polymer used for the formation of the nanofibers.

AB - Synthetic DNA-based oligonucleotides are loaded into porous silicon nanoparticles (pSiNPs) and incorporated into nanofibers of poly(lactide-co-glycolide) (PLGA), poly-l-lactic acid (PLA), or polycaprolactone (PCL). The resulting hybrid nanofibers are characterized for their ability to release the functional oligonucleotide payload under physiologic conditions. Under temperature and pH conditions mimicking physiological values, the PLGA-based nanofibers release >80% of their DNA cargo within 5 days, whereas the PLA and PCL-based fibers require 15 days to release >80% of their cargo. The quantity of DNA released scales with the quantity of DNA-loaded pSiNPs embedded in the nanofibers; mass loadings of between 2.4 and 9.1% (based on mass of DNA-pSiNP construct relative to mass of polymer composite) are investigated. When a responsive DNA-based nanodevice (i.e. molecular beacon) is used as a payload, it retains its functionality during the release period, independent of the polymer used for the formation of the nanofibers.

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Hybrid polymer/porous silicon nanofibers for loading and sustained release of synthetic DNA-based responsive devices

Abstract

Bibliographical note

Funding

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