Hydration of the RNA duplex r(CGCAAAUUUGCG)2 determined by NMR

Maria R. Conte, Graeme L. Conn, Tom Brown, Andrew N. Lane

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The so-called spine of hydration in the minor groove of AnTn tracts in DNA is thought to stabilise the structure, and kinetically bound water detected in the minor groove of such DNA species by NMR has been attributed to a narrow minor groove. We report here an NMR study of hydration of an RNA dodecamer which has a wide, shallow minor groove. Complete assignments of exchangeable protons, and a large number of non-exchangeable protons in r(CGCAAAUUUGCG)2 have been obtained, In addition, ribose C2'-OH resonances have been detected, which are probably involved in hydrogen bonds. Hydration at different sites in the dodecamer has been measured using ROESY and NOESY experiments at 11.75 and 14.1 T. Base protons in both the major and minor grooves are in contact with water, with effective correlation times for the interaction of ~0.5 ns, indicating weak hydration, in contrast to the hydration of adenine C2H in the homologous DNA sequence. NOEs to H1' in the minor groove are consistent with hydration water present that is not observed in the analogous DNA sequence. Hydration kinetics in nucleic acids may be determined by chemical factors such as hydrogen-bonding more than by simple conformational factors such as groove width.

Original languageEnglish
Pages (from-to)3693-3699
Number of pages7
JournalNucleic Acids Research
Volume24
Issue number19
DOIs
StatePublished - 1996

Bibliographical note

Funding Information:
This work was supported by the Medical Research Council of the UK, by a Wellcome Travelling Research Fellowship to MRC and a Royal Society of Edinburgh Caledonian Research Fellowship to GC. We thank Dr J. Feeney for helpful discussions.

ASJC Scopus subject areas

  • Genetics

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