Abstract
Many naturally occurring cells possess an intrinsic ability to cross biological barriers that block conventional drug delivery, and these cells offer a possible mode of active transport across the blood-brain barrier or into the core of tumor masses. While many technologies for the formation of complete, nanoparticle-loaded coatings on cells exist, a complete coating on the cell surface would disrupt the interaction of cells with their environments. To address this issue, cell surface patches that partially cover cell surfaces might provide a superior approach for cell-mediated therapeutic delivery. The goal of this study is to establish a simplified approach to producing polymeric patches of arbitrary shapes on a live cell via surface-mediated photopolymerization. Cell surfaces were nonspecifically labeled with eosin, and polyethylene (glycol) diacrylate (PEGDA) coatings were directed to specific sites using 530 nm irradiation through a chrome-coated photomask. These coatings may entrap drug-loaded or imaging particles. The extent of nonspecific formation of PEGDA hydrogel coatings increased with irradiation time, light intensity, and initiating species; 40 mW/cm2 irradiation for 5 min delivered high-resolution patterns on the surface of A549 cells, and these cells remained viable for 48 h postpatterning with fluorescent nanoparticle-loaded coatings. This work first demonstrated the feasibility of photopatterning polymer patches directly on the surface of cells.
Original language | English |
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Pages (from-to) | 6778-6784 |
Number of pages | 7 |
Journal | Langmuir |
Volume | 33 |
Issue number | 27 |
DOIs | |
State | Published - Jul 11 2017 |
Bibliographical note
Publisher Copyright:© 2017 American Chemical Society.
ASJC Scopus subject areas
- General Materials Science
- Condensed Matter Physics
- Surfaces and Interfaces
- Spectroscopy
- Electrochemistry