Hydroxyl radical generation in the nadh/microsomal reduction of vanadate

Xianglin Shi, N. S. Dalal

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


ESR spin trapping measurements demonstrate generation of hydroxyl (OH) radical from reduction of vanadate by rat liver microsomes/NADH without exogenous H2O2. Catalase decreases the OH signal while increasing a vanadium(4+ signal. Addition of superoxide dismutase (SOD) or measurements under an argon atmosphere show decreased.OH radical production. The results suggest that during the one-electron vanadate reduction process by microsomes/NADH, molecular oxygen is reduced to H2O2, which then reacts with vanadium (4+ to generate.OH radical via a Fenton-like mechanism.

Original languageEnglish
Pages (from-to)369-376
Number of pages8
JournalFree Radical Research
Issue number6
StatePublished - 1992

Bibliographical note

Funding Information:
t This research has been supported by the Department of the Interior's Mineral Institute program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant (31135142 and by NIOSH grant #6O-CCU306149-01-1. $ Author to whom correspondence should be addressed.

Copyright 2016 Elsevier B.V., All rights reserved.


  • Fenton-like mechanism
  • OH radical
  • Vanadate reduction

ASJC Scopus subject areas

  • Biochemistry


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