Hydroxyl radical generation in the nadh/microsomal reduction of vanadate

Xianglin Shi; N. S. Dalal

doi:10.3109/10715769209083141

Hydroxyl radical generation in the nadh/microsomal reduction of vanadate

Xianglin Shi, N. S. Dalal

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

ESR spin trapping measurements demonstrate generation of hydroxyl (OH) radical from reduction of vanadate by rat liver microsomes/NADH without exogenous H₂O₂. Catalase decreases the OH signal while increasing a vanadium(4+ signal. Addition of superoxide dismutase (SOD) or measurements under an argon atmosphere show decreased.OH radical production. The results suggest that during the one-electron vanadate reduction process by microsomes/NADH, molecular oxygen is reduced to H₂O₂, which then reacts with vanadium (4+ to generate.OH radical via a Fenton-like mechanism.

Original language	English
Pages (from-to)	369-376
Number of pages	8
Journal	Free Radical Research
Volume	17
Issue number	6
DOIs	https://doi.org/10.3109/10715769209083141
State	Published - 1992

Bibliographical note

Funding Information:
t This research has been supported by the Department of the Interior's Mineral Institute program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant (31135142 and by NIOSH grant #6O-CCU306149-01-1. $ Author to whom correspondence should be addressed.

Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.

Keywords

Fenton-like mechanism
OH radical
Vanadate reduction

ASJC Scopus subject areas

Biochemistry

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10.3109/10715769209083141

Cite this

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title = "Hydroxyl radical generation in the nadh/microsomal reduction of vanadate",

abstract = "ESR spin trapping measurements demonstrate generation of hydroxyl (OH) radical from reduction of vanadate by rat liver microsomes/NADH without exogenous H2O2. Catalase decreases the OH signal while increasing a vanadium(4+ signal. Addition of superoxide dismutase (SOD) or measurements under an argon atmosphere show decreased.OH radical production. The results suggest that during the one-electron vanadate reduction process by microsomes/NADH, molecular oxygen is reduced to H2O2, which then reacts with vanadium (4+ to generate.OH radical via a Fenton-like mechanism.",

keywords = "Fenton-like mechanism, OH radical, Vanadate reduction",

author = "Xianglin Shi and Dalal, {N. S.}",

note = "Funding Information: t This research has been supported by the Department of the Interior's Mineral Institute program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant (31135142 and by NIOSH grant #6O-CCU306149-01-1. $ Author to whom correspondence should be addressed. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.",

year = "1992",

doi = "10.3109/10715769209083141",

language = "English",

volume = "17",

pages = "369--376",

number = "6",

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TY - JOUR

T1 - Hydroxyl radical generation in the nadh/microsomal reduction of vanadate

AU - Shi, Xianglin

AU - Dalal, N. S.

N1 - Funding Information: t This research has been supported by the Department of the Interior's Mineral Institute program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant (31135142 and by NIOSH grant #6O-CCU306149-01-1. $ Author to whom correspondence should be addressed. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.

PY - 1992

Y1 - 1992

N2 - ESR spin trapping measurements demonstrate generation of hydroxyl (OH) radical from reduction of vanadate by rat liver microsomes/NADH without exogenous H2O2. Catalase decreases the OH signal while increasing a vanadium(4+ signal. Addition of superoxide dismutase (SOD) or measurements under an argon atmosphere show decreased.OH radical production. The results suggest that during the one-electron vanadate reduction process by microsomes/NADH, molecular oxygen is reduced to H2O2, which then reacts with vanadium (4+ to generate.OH radical via a Fenton-like mechanism.

AB - ESR spin trapping measurements demonstrate generation of hydroxyl (OH) radical from reduction of vanadate by rat liver microsomes/NADH without exogenous H2O2. Catalase decreases the OH signal while increasing a vanadium(4+ signal. Addition of superoxide dismutase (SOD) or measurements under an argon atmosphere show decreased.OH radical production. The results suggest that during the one-electron vanadate reduction process by microsomes/NADH, molecular oxygen is reduced to H2O2, which then reacts with vanadium (4+ to generate.OH radical via a Fenton-like mechanism.

KW - Fenton-like mechanism

KW - OH radical

KW - Vanadate reduction

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JF - Free Radical Research

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Hydroxyl radical generation in the nadh/microsomal reduction of vanadate

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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