Hydroxyl Radical-Stressed Whey Protein Isolate: Functional and Rheological Properties

Baohua Kong, Youling L. Xiong, Xuhai Cui, Xinhuai Zhao

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


The objective of the study was to examine the sensitivity of whey protein functionality to oxidizing radicals. Whey protein isolate (WPI) was oxidatively stressed by incubation at 20 °C for 3, 5, and 10 h in hydroxyl radical-generating media containing 0. 1 mM ascorbic acid, 0. 1 mM FeCl3, and 1-10 mM H2O2. Protein solubility decreased (P < 0. 05) with increasing H2O2 concentrations and oxidation time. Surface properties of WPI, including both emulsifying and foaming activities, exhibited significant improvements (P < 0. 05) at H2O2 concentrations up to 5 mM and oxidation time up to 5 h. The longer oxidation time or higher H2O2 concentrations tended to diminish the surface functionality. However, the oxidative stress, though decreasing the onset gelling temperature, had a general detrimental effect on WPI gelation (hardness, springiness, and storage modulus). The results indicated opposing effects of oxidation on WPI: detrimental to hydrodynamic properties (solubility, gelation) but beneficial to surface properties (emulsification, foaming).

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalFood and Bioprocess Technology
Issue number1
StatePublished - Jan 2013

Bibliographical note

Funding Information:
Acknowledgments This study was supported by the National Natural Science Foundation in China (grant no. 30871818) and the Foundation of Innovative Research Team of Higher Education of Heilongjiang Province (grant no. 2010td11).


  • Functional properties
  • Hydroxyl radical
  • Protein oxidation
  • Whey protein isolate

ASJC Scopus subject areas

  • Food Science
  • Safety, Risk, Reliability and Quality
  • Process Chemistry and Technology
  • Industrial and Manufacturing Engineering


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