Hyperspectral techniques in analysis of oral dosage forms

Sara J. Hamilton, Amanda E. Lowell, Robert A. Lodder

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Pharmaceutical oral dosage forms are used in this paper to test the sensitivity and spatial resolution of hyperspectral imaging instruments. The first experiment tested the hypothesis that a near-infrared (IR) tunable diode-based remote sensing system is capable of monitoring degradation of hard gelatin capsules at a relatively long distance (0.5 km). Spectra from the capsules were used to differentiate among capsules exposed to an atmosphere containing 150 ppb formaldehyde for 0, 2, 4, and 8 h. Robust median-based principal component regression with Bayesian inference was employed for outlier detection. The second experiment tested the hypothesis that near-IR imaging spectrometry of tablets permits the identification and composition of multiple individual tablets to be determined simultaneously. A near-IR camera was used to collect thousands of spectra simultaneously from a field of blister-packaged tablets. The number of tablets that a typical near-IR camera can currently analyze simultaneously was estimated to be approximately 1300. The bootstrap error-adjusted single-sample technique chemometric-imaging algorithm was used to draw probability-density contour plots that revealed tablet composition. The single-capsule analysis provides an indication of how far apart the sample and instrumentation can be and still maintain adequate signal-to-noise ratio (S/N), while the multiple-tablet imaging experiment gives an indication of how many samples can be analyzed simultaneously while maintaining an adequate S/N and pixel coverage on each sample.

Original languageEnglish
Pages (from-to)561-570
Number of pages10
JournalJournal of Biomedical Optics
Issue number4
StatePublished - Oct 2002

Bibliographical note

Funding Information:
The authors acknowledge the support of the National Science Foundation through CHE-9257998 and DGE-9870691. A preliminary report on this work was published in the Proc. SPIE Symp. BiOS., 2002.


  • Capsule
  • Gelatin
  • Near infrared
  • Tablet
  • Tunable laser

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering


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