Abstract
Hypoxanthine phosphoribosyltransferase (HPRT) is a purine salvage enzyme that catalyzes conversion of hypoxanthine and guanine to their respective mononucleotides. Because of its ubiquitous nature, HPRT is known as a "housekeeping" gene and has been frequently used as an internal control in reverse transcriptase-polymerase chain reaction (RT-PCR) quantification of cytokine mRNA. Cloning and sequencing of the gerbil HPRT cDNA sequence is an important step in the development of RT-PCR procedures in this model. Two forms of gerbil HPRT cDNA were isolated and molecularly characterized.
| Original language | English |
|---|---|
| Pages (from-to) | 179-183 |
| Number of pages | 5 |
| Journal | Laboratory Animal Science |
| Volume | 48 |
| Issue number | 2 |
| State | Published - 1998 |
ASJC Scopus subject areas
- Animal Science and Zoology
- Veterinary (all)