Hypoxanthine Phosphoribosyltransferase cDNA in Gerbils (Meriones unguiculatus)

Zhuming Mai; David W. Horohov; Thomas R. Klei

Hypoxanthine Phosphoribosyltransferase cDNA in Gerbils (Meriones unguiculatus)

Zhuming Mai, David W. Horohov, Thomas R. Klei

Veterinary Science

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Hypoxanthine phosphoribosyltransferase (HPRT) is a purine salvage enzyme that catalyzes conversion of hypoxanthine and guanine to their respective mononucleotides. Because of its ubiquitous nature, HPRT is known as a "housekeeping" gene and has been frequently used as an internal control in reverse transcriptase-polymerase chain reaction (RT-PCR) quantification of cytokine mRNA. Cloning and sequencing of the gerbil HPRT cDNA sequence is an important step in the development of RT-PCR procedures in this model. Two forms of gerbil HPRT cDNA were isolated and molecularly characterized.

Original language	English
Pages (from-to)	179-183
Number of pages	5
Journal	Laboratory Animal Science
Volume	48
Issue number	2
State	Published - 1998

ASJC Scopus subject areas

Animal Science and Zoology
General Veterinary

Cite this

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abstract = "Hypoxanthine phosphoribosyltransferase (HPRT) is a purine salvage enzyme that catalyzes conversion of hypoxanthine and guanine to their respective mononucleotides. Because of its ubiquitous nature, HPRT is known as a {"}housekeeping{"} gene and has been frequently used as an internal control in reverse transcriptase-polymerase chain reaction (RT-PCR) quantification of cytokine mRNA. Cloning and sequencing of the gerbil HPRT cDNA sequence is an important step in the development of RT-PCR procedures in this model. Two forms of gerbil HPRT cDNA were isolated and molecularly characterized.",

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T1 - Hypoxanthine Phosphoribosyltransferase cDNA in Gerbils (Meriones unguiculatus)

AU - Mai, Zhuming

AU - Horohov, David W.

AU - Klei, Thomas R.

PY - 1998

Y1 - 1998

N2 - Hypoxanthine phosphoribosyltransferase (HPRT) is a purine salvage enzyme that catalyzes conversion of hypoxanthine and guanine to their respective mononucleotides. Because of its ubiquitous nature, HPRT is known as a "housekeeping" gene and has been frequently used as an internal control in reverse transcriptase-polymerase chain reaction (RT-PCR) quantification of cytokine mRNA. Cloning and sequencing of the gerbil HPRT cDNA sequence is an important step in the development of RT-PCR procedures in this model. Two forms of gerbil HPRT cDNA were isolated and molecularly characterized.

AB - Hypoxanthine phosphoribosyltransferase (HPRT) is a purine salvage enzyme that catalyzes conversion of hypoxanthine and guanine to their respective mononucleotides. Because of its ubiquitous nature, HPRT is known as a "housekeeping" gene and has been frequently used as an internal control in reverse transcriptase-polymerase chain reaction (RT-PCR) quantification of cytokine mRNA. Cloning and sequencing of the gerbil HPRT cDNA sequence is an important step in the development of RT-PCR procedures in this model. Two forms of gerbil HPRT cDNA were isolated and molecularly characterized.

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C2 - 10090010

AN - SCOPUS:0031920631

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VL - 48

SP - 179

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JO - Laboratory Animal Science

JF - Laboratory Animal Science

IS - 2

ER -

Hypoxanthine Phosphoribosyltransferase cDNA in Gerbils (Meriones unguiculatus)

Abstract

ASJC Scopus subject areas

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