Identification and characterization of two distinctive RNA binding activities in pea nuclear extracts

Jaydip Das Gupta, Qingshun Li, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


We have identified and purified two RNA binding factors from pea nuclear extracts. One factor (RBF-1) consisted of at least two polypeptides with molecular weights of approximately 27 and 59 kD; each of these polypeptides could be crosslinked to labelled RNA by ultraviolet light. The other factor (RBF-2) also consisted of two polypeptides (of approximately 93 and 126 kD in size) that could be crosslinked to RNA by UV. Both factors showed general preferences for single stranded RNA. However, RBF-1 displayed a preference for poly(A) and poly(U) over poly(G) or poly(C), while RBF-2 had a strong preference for poly(U) over the other three homopolymers. These properties are suggestive of possible roles for these factors in RNA metabolism in plant nuclei.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalJournal of Plant Biochemistry and Biotechnology
Issue number1
StatePublished - Jan 1998

Bibliographical note

Funding Information:
I am greatly indebted to Dr. E. Orias for many constructive discussions on the model of copy number control for macronuclear fragments and to a number of colleagues who assisted with constructive criticism of this manuscript and who communicated the results of their investigations prior to their publication. In particular, 1 wish to thank Drs. S. Allen, E. Blackburn, K. Conover, K. Jones, V. Merriam, E. Orias, R. Pearlman. and M.-C. Yao. Experimental work in the author’s laboratory was supported by research grants from the National Science Foundation (PCM-8215666) and the Academic Senate of the University of California (2455).


  • Binding activities
  • Nuclear extracts
  • Pea
  • RNA

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science


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