Identification and characterization of viral defective RNA genomes in influenza B virus

Zizhang Sheng; Runxia Liu; Jieshi Yu; Zhiguang Ran; Simon J. Newkirk; Wenfeng An; Feng Li; Dan Wang

doi:10.1099/jgv.0.001018

Identification and characterization of viral defective RNA genomes in influenza B virus

Zizhang Sheng, Runxia Liu, Jieshi Yu, Zhiguang Ran, Simon J. Newkirk, Wenfeng An, Feng Li, Dan Wang

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Influenza B virus (FLUBV) is an important pathogen that infects humans and causes seasonal influenza epidemics. To date, little is known about defective genomes of FLUBV and their roles in viral replication. In this study, by using a next-generation sequencing approach, we analyzed total mRNAs extracted from A549 cells infected with B/Brisbane/60/2008 virus (Victoria lineage), and identified four defective FLUBV genomes with two (PB1ΔA and PB1ΔB) from the polymerase basic subunit 1 (PB1) segment and the other two (MΔA and MΔB) from the matrix (M) protein-encoding segment. These defective genomes contained significant deletions in the central regions with each having the potential for encoding a novel polypeptide. Significantly, each of the discovered defective RNAs can potently inhibit the replication of B/Yamanashi/166/98 (Yamagata lineage). Furthermore, PB1ΔA was able to interfere modestly with influenza A virus (FLUAV) replication. In summary, our study provides important initial insights into FLUBV defective-interfering genomes, which can be further explored to achieve better understanding of the replication, pathogenesis and evolution of FLUBV.

Original language	English
Pages (from-to)	475-488
Number of pages	14
Journal	Journal of General Virology
Volume	99
Issue number	4
DOIs	https://doi.org/10.1099/jgv.0.001018
State	Published - Apr 2018

Bibliographical note

Publisher Copyright:
© 2018 The Authors.

Funding

The study was funded by South Dakota Agricultural Experiment Station (3AH-477 to F. L.), the National Science Foundation/EPSCoR Cooperative Agreement #IIA-1355423, the South Dakota Research and Innovation Center, and BioSNTR, and NIH AI121906 (sub-award to D. W.).

Funders	Funder number
NIH AI121906
National Science Foundation/EPSCoR	-1355423
National Science Foundation (NSF)	#IIA-1355423
National Institutes of Health (NIH)
National Institute of Allergy and Infectious Diseases	R21AI121906
South Dakota Agricultural Experiment Station	3AH-477

Keywords

Defective RNA genomes
Influenza B virus
Interfering

ASJC Scopus subject areas

Virology

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10.1099/jgv.0.001018

Cite this

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title = "Identification and characterization of viral defective RNA genomes in influenza B virus",

abstract = "Influenza B virus (FLUBV) is an important pathogen that infects humans and causes seasonal influenza epidemics. To date, little is known about defective genomes of FLUBV and their roles in viral replication. In this study, by using a next-generation sequencing approach, we analyzed total mRNAs extracted from A549 cells infected with B/Brisbane/60/2008 virus (Victoria lineage), and identified four defective FLUBV genomes with two (PB1ΔA and PB1ΔB) from the polymerase basic subunit 1 (PB1) segment and the other two (MΔA and MΔB) from the matrix (M) protein-encoding segment. These defective genomes contained significant deletions in the central regions with each having the potential for encoding a novel polypeptide. Significantly, each of the discovered defective RNAs can potently inhibit the replication of B/Yamanashi/166/98 (Yamagata lineage). Furthermore, PB1ΔA was able to interfere modestly with influenza A virus (FLUAV) replication. In summary, our study provides important initial insights into FLUBV defective-interfering genomes, which can be further explored to achieve better understanding of the replication, pathogenesis and evolution of FLUBV.",

keywords = "Defective RNA genomes, Influenza B virus, Interfering",

author = "Zizhang Sheng and Runxia Liu and Jieshi Yu and Zhiguang Ran and Newkirk, {Simon J.} and Wenfeng An and Feng Li and Dan Wang",

note = "Publisher Copyright: {\textcopyright} 2018 The Authors.",

year = "2018",

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doi = "10.1099/jgv.0.001018",

language = "English",

volume = "99",

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T1 - Identification and characterization of viral defective RNA genomes in influenza B virus

AU - Sheng, Zizhang

AU - Liu, Runxia

AU - Yu, Jieshi

AU - Ran, Zhiguang

AU - Newkirk, Simon J.

AU - An, Wenfeng

AU - Li, Feng

AU - Wang, Dan

PY - 2018/4

Y1 - 2018/4

N2 - Influenza B virus (FLUBV) is an important pathogen that infects humans and causes seasonal influenza epidemics. To date, little is known about defective genomes of FLUBV and their roles in viral replication. In this study, by using a next-generation sequencing approach, we analyzed total mRNAs extracted from A549 cells infected with B/Brisbane/60/2008 virus (Victoria lineage), and identified four defective FLUBV genomes with two (PB1ΔA and PB1ΔB) from the polymerase basic subunit 1 (PB1) segment and the other two (MΔA and MΔB) from the matrix (M) protein-encoding segment. These defective genomes contained significant deletions in the central regions with each having the potential for encoding a novel polypeptide. Significantly, each of the discovered defective RNAs can potently inhibit the replication of B/Yamanashi/166/98 (Yamagata lineage). Furthermore, PB1ΔA was able to interfere modestly with influenza A virus (FLUAV) replication. In summary, our study provides important initial insights into FLUBV defective-interfering genomes, which can be further explored to achieve better understanding of the replication, pathogenesis and evolution of FLUBV.

AB - Influenza B virus (FLUBV) is an important pathogen that infects humans and causes seasonal influenza epidemics. To date, little is known about defective genomes of FLUBV and their roles in viral replication. In this study, by using a next-generation sequencing approach, we analyzed total mRNAs extracted from A549 cells infected with B/Brisbane/60/2008 virus (Victoria lineage), and identified four defective FLUBV genomes with two (PB1ΔA and PB1ΔB) from the polymerase basic subunit 1 (PB1) segment and the other two (MΔA and MΔB) from the matrix (M) protein-encoding segment. These defective genomes contained significant deletions in the central regions with each having the potential for encoding a novel polypeptide. Significantly, each of the discovered defective RNAs can potently inhibit the replication of B/Yamanashi/166/98 (Yamagata lineage). Furthermore, PB1ΔA was able to interfere modestly with influenza A virus (FLUAV) replication. In summary, our study provides important initial insights into FLUBV defective-interfering genomes, which can be further explored to achieve better understanding of the replication, pathogenesis and evolution of FLUBV.

KW - Defective RNA genomes

KW - Influenza B virus

KW - Interfering

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Identification and characterization of viral defective RNA genomes in influenza B virus

Abstract

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Funding

Keywords

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