TY - JOUR
T1 - Identification and characterization of viral defective RNA genomes in influenza B virus
AU - Sheng, Zizhang
AU - Liu, Runxia
AU - Yu, Jieshi
AU - Ran, Zhiguang
AU - Newkirk, Simon J.
AU - An, Wenfeng
AU - Li, Feng
AU - Wang, Dan
N1 - Publisher Copyright:
© 2018 The Authors.
PY - 2018/4
Y1 - 2018/4
N2 - Influenza B virus (FLUBV) is an important pathogen that infects humans and causes seasonal influenza epidemics. To date, little is known about defective genomes of FLUBV and their roles in viral replication. In this study, by using a next-generation sequencing approach, we analyzed total mRNAs extracted from A549 cells infected with B/Brisbane/60/2008 virus (Victoria lineage), and identified four defective FLUBV genomes with two (PB1ΔA and PB1ΔB) from the polymerase basic subunit 1 (PB1) segment and the other two (MΔA and MΔB) from the matrix (M) protein-encoding segment. These defective genomes contained significant deletions in the central regions with each having the potential for encoding a novel polypeptide. Significantly, each of the discovered defective RNAs can potently inhibit the replication of B/Yamanashi/166/98 (Yamagata lineage). Furthermore, PB1ΔA was able to interfere modestly with influenza A virus (FLUAV) replication. In summary, our study provides important initial insights into FLUBV defective-interfering genomes, which can be further explored to achieve better understanding of the replication, pathogenesis and evolution of FLUBV.
AB - Influenza B virus (FLUBV) is an important pathogen that infects humans and causes seasonal influenza epidemics. To date, little is known about defective genomes of FLUBV and their roles in viral replication. In this study, by using a next-generation sequencing approach, we analyzed total mRNAs extracted from A549 cells infected with B/Brisbane/60/2008 virus (Victoria lineage), and identified four defective FLUBV genomes with two (PB1ΔA and PB1ΔB) from the polymerase basic subunit 1 (PB1) segment and the other two (MΔA and MΔB) from the matrix (M) protein-encoding segment. These defective genomes contained significant deletions in the central regions with each having the potential for encoding a novel polypeptide. Significantly, each of the discovered defective RNAs can potently inhibit the replication of B/Yamanashi/166/98 (Yamagata lineage). Furthermore, PB1ΔA was able to interfere modestly with influenza A virus (FLUAV) replication. In summary, our study provides important initial insights into FLUBV defective-interfering genomes, which can be further explored to achieve better understanding of the replication, pathogenesis and evolution of FLUBV.
KW - Defective RNA genomes
KW - Influenza B virus
KW - Interfering
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U2 - 10.1099/jgv.0.001018
DO - 10.1099/jgv.0.001018
M3 - Article
C2 - 29458654
AN - SCOPUS:85045119149
SN - 0022-1317
VL - 99
SP - 475
EP - 488
JO - Journal of General Virology
JF - Journal of General Virology
IS - 4
ER -