Identification and developmental expression of the mitochondrial phosphate transport protein gene from the spruce budworm, Choristoneura fumiferana

Qi Li Feng; Tim R. Ladd; Arthur Retnakaran; Kenneth G. Davey; Subba R. Palli

doi:10.1016/S0965-1748(98)00055-1

Identification and developmental expression of the mitochondrial phosphate transport protein gene from the spruce budworm, Choristoneura fumiferana

Qi Li Feng, Tim R. Ladd, Arthur Retnakaran, Kenneth G. Davey, Subba R. Palli

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Phosphate transport protein (PTP) is a mitochondrial inner membrane protein responsible for the translocation of inorganic phosphate into the mitochondrial matrix. A full length cDNA clone encoding the PTP was isolated from the spruce budworm, Choristoneura fumiferana. The deduced amino acid sequence of the longest ORF of CfPTP cDNA showed high similarity with the amino acid sequences of PTPs cloned from several species. Phylogenetic tree analysis indicated that CfPTP occupied an intermediate position between vertebrates on the one side and yeast and nematodes on the other side. Studies on the developmental expression of CfPTP mRNA showed that higher levels of mRNA were present during the feeding and growing stages than during molting periods.

Original language	English
Pages (from-to)	791-799
Number of pages	9
Journal	Insect Biochemistry and Molecular Biology
Volume	28
Issue number	10
DOIs	https://doi.org/10.1016/S0965-1748(98)00055-1
State	Published - Oct 1998

Bibliographical note

Funding Information:
This research is supported in part by a grant from the Natural Science and Engineering Research Council to K.G. Davey and by the National Biotechnology Strategy Fund, and the Science and Technology Opportunities Fund of Canadian Forest Service to S.R. Palli.

Keywords

CDNA cloning
Gene expression
Membrane transport
Mitochondria
Molting
Phosphate carrier

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Insect Science

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10.1016/S0965-1748(98)00055-1

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title = "Identification and developmental expression of the mitochondrial phosphate transport protein gene from the spruce budworm, Choristoneura fumiferana",

abstract = "Phosphate transport protein (PTP) is a mitochondrial inner membrane protein responsible for the translocation of inorganic phosphate into the mitochondrial matrix. A full length cDNA clone encoding the PTP was isolated from the spruce budworm, Choristoneura fumiferana. The deduced amino acid sequence of the longest ORF of CfPTP cDNA showed high similarity with the amino acid sequences of PTPs cloned from several species. Phylogenetic tree analysis indicated that CfPTP occupied an intermediate position between vertebrates on the one side and yeast and nematodes on the other side. Studies on the developmental expression of CfPTP mRNA showed that higher levels of mRNA were present during the feeding and growing stages than during molting periods.",

keywords = "CDNA cloning, Gene expression, Membrane transport, Mitochondria, Molting, Phosphate carrier",

author = "Feng, {Qi Li} and Ladd, {Tim R.} and Arthur Retnakaran and Davey, {Kenneth G.} and Palli, {Subba R.}",

note = "Funding Information: This research is supported in part by a grant from the Natural Science and Engineering Research Council to K.G. Davey and by the National Biotechnology Strategy Fund, and the Science and Technology Opportunities Fund of Canadian Forest Service to S.R. Palli.",

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AU - Feng, Qi Li

AU - Ladd, Tim R.

AU - Retnakaran, Arthur

AU - Davey, Kenneth G.

AU - Palli, Subba R.

N1 - Funding Information: This research is supported in part by a grant from the Natural Science and Engineering Research Council to K.G. Davey and by the National Biotechnology Strategy Fund, and the Science and Technology Opportunities Fund of Canadian Forest Service to S.R. Palli.

PY - 1998/10

Y1 - 1998/10

N2 - Phosphate transport protein (PTP) is a mitochondrial inner membrane protein responsible for the translocation of inorganic phosphate into the mitochondrial matrix. A full length cDNA clone encoding the PTP was isolated from the spruce budworm, Choristoneura fumiferana. The deduced amino acid sequence of the longest ORF of CfPTP cDNA showed high similarity with the amino acid sequences of PTPs cloned from several species. Phylogenetic tree analysis indicated that CfPTP occupied an intermediate position between vertebrates on the one side and yeast and nematodes on the other side. Studies on the developmental expression of CfPTP mRNA showed that higher levels of mRNA were present during the feeding and growing stages than during molting periods.

AB - Phosphate transport protein (PTP) is a mitochondrial inner membrane protein responsible for the translocation of inorganic phosphate into the mitochondrial matrix. A full length cDNA clone encoding the PTP was isolated from the spruce budworm, Choristoneura fumiferana. The deduced amino acid sequence of the longest ORF of CfPTP cDNA showed high similarity with the amino acid sequences of PTPs cloned from several species. Phylogenetic tree analysis indicated that CfPTP occupied an intermediate position between vertebrates on the one side and yeast and nematodes on the other side. Studies on the developmental expression of CfPTP mRNA showed that higher levels of mRNA were present during the feeding and growing stages than during molting periods.

KW - CDNA cloning

KW - Gene expression

KW - Membrane transport

KW - Mitochondria

KW - Molting

KW - Phosphate carrier

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Identification and developmental expression of the mitochondrial phosphate transport protein gene from the spruce budworm, Choristoneura fumiferana

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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