Identification, expression analysis, genomic organization and cellular location of a novel protein with a RhoGEF domain

Sze Wah Tse; Jennifer A. Broderick; Mei Ling Wei; Min Hua Luo; Deborah Smith; Peter McCaffery; Stefan Stamm; Athena Andreadis

doi:10.1016/j.gene.2005.06.025

Identification, expression analysis, genomic organization and cellular location of a novel protein with a RhoGEF domain

Sze Wah Tse, Jennifer A. Broderick, Mei Ling Wei, Min Hua Luo, Deborah Smith, Peter McCaffery, Stefan Stamm, Athena Andreadis

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

In this study we describe the identification and characterization of a novel cytosolic protein of the guanine exchange factor (GEF) family. The human cDNA corresponds to predicted human protein FLJ00128/FLJ10357 located on chromosome 14q11.2. The deduced protein sequence contains in its C-terminus a RhoGEF domain followed by a pleckstrin domain. Its N-terminus, central region and RhoGEF/pleckstrin domain are homologous to the recently identified zebrafish Quattro protein, which is involved in morphogenetic movements mediated by the actin cytoskeleton. Based on the homology of our protein's RhoGEF domain to the RhoGEF domains of Trio, Duo and Duet and its homology with Quattro, we named it Solo. The Solo mRNA is ubiquitously expressed but enriched in brain, its expression peaks perinatally and it undergoes extensive alternative splicing. In both myoblasts and neuroblastoma cells, the Solo protein is concentrated around the nucleus.

Original language	English
Pages (from-to)	63-72
Number of pages	10
Journal	Gene
Volume	359
Issue number	1-2
DOIs	https://doi.org/10.1016/j.gene.2005.06.025
State	Published - Oct 10 2005

Bibliographical note

Funding Information:
This study was supported by NICHD grant HD05515 to A.A. and P.M. We wish to thank John Memmott for expert technical assistance.

Funding

This study was supported by NICHD grant HD05515 to A.A. and P.M. We wish to thank John Memmott for expert technical assistance.

Funders	Funder number
Eunice Kennedy Shriver National Institute of Child Health and Human Development	P01HD005515

Keywords

Alternative splicing
FLJ protein
Perinuclear localization
Yeast two-hybrid screen

ASJC Scopus subject areas

Genetics

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10.1016/j.gene.2005.06.025

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title = "Identification, expression analysis, genomic organization and cellular location of a novel protein with a RhoGEF domain",

abstract = "In this study we describe the identification and characterization of a novel cytosolic protein of the guanine exchange factor (GEF) family. The human cDNA corresponds to predicted human protein FLJ00128/FLJ10357 located on chromosome 14q11.2. The deduced protein sequence contains in its C-terminus a RhoGEF domain followed by a pleckstrin domain. Its N-terminus, central region and RhoGEF/pleckstrin domain are homologous to the recently identified zebrafish Quattro protein, which is involved in morphogenetic movements mediated by the actin cytoskeleton. Based on the homology of our protein's RhoGEF domain to the RhoGEF domains of Trio, Duo and Duet and its homology with Quattro, we named it Solo. The Solo mRNA is ubiquitously expressed but enriched in brain, its expression peaks perinatally and it undergoes extensive alternative splicing. In both myoblasts and neuroblastoma cells, the Solo protein is concentrated around the nucleus.",

keywords = "Alternative splicing, FLJ protein, Perinuclear localization, Yeast two-hybrid screen",

author = "Tse, {Sze Wah} and Broderick, {Jennifer A.} and Wei, {Mei Ling} and Luo, {Min Hua} and Deborah Smith and Peter McCaffery and Stefan Stamm and Athena Andreadis",

note = "Funding Information: This study was supported by NICHD grant HD05515 to A.A. and P.M. We wish to thank John Memmott for expert technical assistance.",

year = "2005",

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doi = "10.1016/j.gene.2005.06.025",

language = "English",

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AU - Tse, Sze Wah

AU - Broderick, Jennifer A.

AU - Wei, Mei Ling

AU - Luo, Min Hua

AU - Smith, Deborah

AU - McCaffery, Peter

AU - Stamm, Stefan

AU - Andreadis, Athena

N1 - Funding Information: This study was supported by NICHD grant HD05515 to A.A. and P.M. We wish to thank John Memmott for expert technical assistance.

PY - 2005/10/10

Y1 - 2005/10/10

N2 - In this study we describe the identification and characterization of a novel cytosolic protein of the guanine exchange factor (GEF) family. The human cDNA corresponds to predicted human protein FLJ00128/FLJ10357 located on chromosome 14q11.2. The deduced protein sequence contains in its C-terminus a RhoGEF domain followed by a pleckstrin domain. Its N-terminus, central region and RhoGEF/pleckstrin domain are homologous to the recently identified zebrafish Quattro protein, which is involved in morphogenetic movements mediated by the actin cytoskeleton. Based on the homology of our protein's RhoGEF domain to the RhoGEF domains of Trio, Duo and Duet and its homology with Quattro, we named it Solo. The Solo mRNA is ubiquitously expressed but enriched in brain, its expression peaks perinatally and it undergoes extensive alternative splicing. In both myoblasts and neuroblastoma cells, the Solo protein is concentrated around the nucleus.

AB - In this study we describe the identification and characterization of a novel cytosolic protein of the guanine exchange factor (GEF) family. The human cDNA corresponds to predicted human protein FLJ00128/FLJ10357 located on chromosome 14q11.2. The deduced protein sequence contains in its C-terminus a RhoGEF domain followed by a pleckstrin domain. Its N-terminus, central region and RhoGEF/pleckstrin domain are homologous to the recently identified zebrafish Quattro protein, which is involved in morphogenetic movements mediated by the actin cytoskeleton. Based on the homology of our protein's RhoGEF domain to the RhoGEF domains of Trio, Duo and Duet and its homology with Quattro, we named it Solo. The Solo mRNA is ubiquitously expressed but enriched in brain, its expression peaks perinatally and it undergoes extensive alternative splicing. In both myoblasts and neuroblastoma cells, the Solo protein is concentrated around the nucleus.

KW - Alternative splicing

KW - FLJ protein

KW - Perinuclear localization

KW - Yeast two-hybrid screen

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Identification, expression analysis, genomic organization and cellular location of a novel protein with a RhoGEF domain

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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