Identification of a dominant gene in Medicago truncatula that restricts nodulation by Sinorhizobium meliloti strain Rm41

Jinge Liu, Shengming Yang, Qiaolin Zheng, Hongyan Zhu

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Background: Leguminous plants are able to form a root nodule symbiosis with nitrogen-fixing soil bacteria called rhizobia. This symbiotic association shows a high level of specificity. Beyond the specificity for the legume family, individual legume species/genotypes can only interact with certain restricted group of bacterial species or strains. Specificity in this system is regulated by complex signal exchange between the two symbiotic partners and thus multiple genetic mechanisms could be involved in the recognition process. Knowledge of the molecular mechanisms controlling symbiotic specificity could enable genetic improvement of legume nitrogen fixation, and may also reveal the possible mechanisms that restrict root nodule symbiosis in non-legumes.Results: We screened a core collection of Medicago truncatula genotypes with several strains of Sinorhizobium meliloti and identified a naturally occurring dominant gene that restricts nodulation by S. meliloti Rm41. We named this gene as Mt-NS1 (for M.truncatulanodulation specificity 1). We have mapped the Mt-NS1 locus within a small genomic region on M. truncatula chromosome 8. The data reported here will facilitate positional cloning of the Mt-NS1 gene.Conclusions: Evolution of symbiosis specificity involves both rhizobial and host genes. From the bacterial side, specificity determinants include Nod factors, surface polysaccharides, and secreted proteins. However, we know relatively less from the host side. We recently demonstrated that a component of this specificity in soybeans is defined by plant NBS-LRR resistance (R) genes that recognize effector proteins delivered by the type III secretion system (T3SS) of the rhizobial symbionts. However, the lack of a T3SS in many sequenced S. meliloti strains raises the question of how the specificity is regulated in the Medicago-Sinorhizobium system beyond Nod-factor perception. Thus, cloning and characterization of Mt-NS1 will add a new dimension to our knowledge about the genetic control of nodulation specificity in the legume-rhizobial symbiosis.

Original languageEnglish
Article number167
JournalBMC Plant Biology
Volume14
Issue number1
DOIs
StatePublished - Jun 16 2014

Bibliographical note

Funding Information:
We thank Drs. Bradley L. Reuhs (Purdue University), Kathryn M. Jones (Florida State University), and Peter Putnoky (University of Pecs, Hungary) for providing the S. meliloti strains used in this study. We also thank Dr. Jean-Marie Prosperi (INRA, France) for providing M. truncatula seeds. This research was supported by a grant from Kentucky Science and Engineering Foundation and a grant from United States Department of Agriculture-Agriculture and Food Research Initiative (to HZ).

Keywords

  • Legume
  • Medicago truncatula
  • Nitrogen fixation
  • Nodulation specificity

ASJC Scopus subject areas

  • Plant Science

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