Abstract
Long non-coding RNAs (lncRNA) are thought to be important epigenetic regulators involved in the development of a variety of cancers. Alterations in lncRNA expression are associated with exposure to chemical carcinogens. However, it is still unclear whether lncRNA expression during lung carcinogenesis is induced by chemical carcinogens. In this study, using NNK-induced rat lung cancer model established by our previous study, we determined the lncRNA expression profiles, and an alteration in lncRNA expression was observed in lung cancer tissues and blood in the NNK treatment group. Using quantitative reverse-transcription PCR (qRT-PCR), five differentially expressed lncRNAs were further detected and validated. We identified a novel lncRNA, NR_026689, which showed increased expression in lung cancer tissues induced by NNK and the alteration of lncRNA NR_026689 was specifically observed in lung tissue. The level of NR_026689 was determined and significantly increased in rat whole blood at the 10th and 20th week after NNK treatment to evaluate it as a potential early marker for lung cancer. Together, these findings suggest that lncRNA NR_026689 may be a potential early biomarker for lung cancer and is associated with lung carcinogenesis induced by NNK.
| Original language | English |
|---|---|
| Pages (from-to) | 14486-14498 |
| Number of pages | 13 |
| Journal | Oncotarget |
| Volume | 7 |
| Issue number | 12 |
| DOIs | |
| State | Published - Mar 22 2016 |
Bibliographical note
Funding Information:This work was supported by the National Natural Science Foundation of China (81172633 to J.Y., 21277036 to J.Y., 81473000 to W.J.), the Research Fund for the Doctoral Program of Higher Education of China (20114423110002 to J.Y.), the Guangdong Natural Science Foundation (2014A030311017 to J.Y.), the University Talent Program of Guangdong (2013-164 to J.Y.), the University Major Program of Guangdong (2014SZD48).
Keywords
- LncRNA
- Lung carcinogenesis
- NNK
- NR_026689
ASJC Scopus subject areas
- Oncology
