Identification of a long non-coding RNA NR_026689 associated with lung carcinogenesis induced by NNK

Jianjun Wu; Xun Li; Yiqin Xu; Ti Yang; Qiaoyuan Yang; Chengfeng Yang; Yiguo Jiang

doi:10.18632/oncotarget.7475

Identification of a long non-coding RNA NR_026689 associated with lung carcinogenesis induced by NNK

Jianjun Wu
, Xun Li
, Yiqin Xu
, Ti Yang
, Qiaoyuan Yang
, Chengfeng Yang
, Yiguo Jiang

Markey Cancer Center / Cancer Research Priority Initiative

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Long non-coding RNAs (lncRNA) are thought to be important epigenetic regulators involved in the development of a variety of cancers. Alterations in lncRNA expression are associated with exposure to chemical carcinogens. However, it is still unclear whether lncRNA expression during lung carcinogenesis is induced by chemical carcinogens. In this study, using NNK-induced rat lung cancer model established by our previous study, we determined the lncRNA expression profiles, and an alteration in lncRNA expression was observed in lung cancer tissues and blood in the NNK treatment group. Using quantitative reverse-transcription PCR (qRT-PCR), five differentially expressed lncRNAs were further detected and validated. We identified a novel lncRNA, NR_026689, which showed increased expression in lung cancer tissues induced by NNK and the alteration of lncRNA NR_026689 was specifically observed in lung tissue. The level of NR_026689 was determined and significantly increased in rat whole blood at the 10th and 20th week after NNK treatment to evaluate it as a potential early marker for lung cancer. Together, these findings suggest that lncRNA NR_026689 may be a potential early biomarker for lung cancer and is associated with lung carcinogenesis induced by NNK.

Original language	English
Pages (from-to)	14486-14498
Number of pages	13
Journal	Oncotarget
Volume	7
Issue number	12
DOIs	https://doi.org/10.18632/oncotarget.7475
State	Published - Mar 22 2016

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (81172633 to J.Y., 21277036 to J.Y., 81473000 to W.J.), the Research Fund for the Doctoral Program of Higher Education of China (20114423110002 to J.Y.), the Guangdong Natural Science Foundation (2014A030311017 to J.Y.), the University Talent Program of Guangdong (2013-164 to J.Y.), the University Major Program of Guangdong (2014SZD48).

Funding

This work was supported by the National Natural Science Foundation of China (81172633 to J.Y., 21277036 to J.Y., 81473000 to W.J.), the Research Fund for the Doctoral Program of Higher Education of China (20114423110002 to J.Y.), the Guangdong Natural Science Foundation (2014A030311017 to J.Y.), the University Talent Program of Guangdong (2013-164 to J.Y.), the University Major Program of Guangdong (2014SZD48).

Funders	Funder number
University Major Program of Guangdong	2014SZD48
University Talent Program of Guangdong	2013-164
National Natural Science Foundation of China (NSFC)	81473000, 81172633, 21277036
Natural Science Foundation of Guangdong Province	2014A030311017
Specialized Research Fund for the Doctoral Program of Higher Education of China	20114423110002

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

LncRNA
Lung carcinogenesis
NNK
NR_026689

ASJC Scopus subject areas

Oncology

Access to Document

10.18632/oncotarget.7475

Cite this

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title = "Identification of a long non-coding RNA NR\_026689 associated with lung carcinogenesis induced by NNK",

abstract = "Long non-coding RNAs (lncRNA) are thought to be important epigenetic regulators involved in the development of a variety of cancers. Alterations in lncRNA expression are associated with exposure to chemical carcinogens. However, it is still unclear whether lncRNA expression during lung carcinogenesis is induced by chemical carcinogens. In this study, using NNK-induced rat lung cancer model established by our previous study, we determined the lncRNA expression profiles, and an alteration in lncRNA expression was observed in lung cancer tissues and blood in the NNK treatment group. Using quantitative reverse-transcription PCR (qRT-PCR), five differentially expressed lncRNAs were further detected and validated. We identified a novel lncRNA, NR\_026689, which showed increased expression in lung cancer tissues induced by NNK and the alteration of lncRNA NR\_026689 was specifically observed in lung tissue. The level of NR\_026689 was determined and significantly increased in rat whole blood at the 10th and 20th week after NNK treatment to evaluate it as a potential early marker for lung cancer. Together, these findings suggest that lncRNA NR\_026689 may be a potential early biomarker for lung cancer and is associated with lung carcinogenesis induced by NNK.",

keywords = "LncRNA, Lung carcinogenesis, NNK, NR\_026689",

author = "Jianjun Wu and Xun Li and Yiqin Xu and Ti Yang and Qiaoyuan Yang and Chengfeng Yang and Yiguo Jiang",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (81172633 to J.Y., 21277036 to J.Y., 81473000 to W.J.), the Research Fund for the Doctoral Program of Higher Education of China (20114423110002 to J.Y.), the Guangdong Natural Science Foundation (2014A030311017 to J.Y.), the University Talent Program of Guangdong (2013-164 to J.Y.), the University Major Program of Guangdong (2014SZD48).",

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AU - Yang, Ti

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AU - Yang, Chengfeng

AU - Jiang, Yiguo

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Y1 - 2016/3/22

N2 - Long non-coding RNAs (lncRNA) are thought to be important epigenetic regulators involved in the development of a variety of cancers. Alterations in lncRNA expression are associated with exposure to chemical carcinogens. However, it is still unclear whether lncRNA expression during lung carcinogenesis is induced by chemical carcinogens. In this study, using NNK-induced rat lung cancer model established by our previous study, we determined the lncRNA expression profiles, and an alteration in lncRNA expression was observed in lung cancer tissues and blood in the NNK treatment group. Using quantitative reverse-transcription PCR (qRT-PCR), five differentially expressed lncRNAs were further detected and validated. We identified a novel lncRNA, NR_026689, which showed increased expression in lung cancer tissues induced by NNK and the alteration of lncRNA NR_026689 was specifically observed in lung tissue. The level of NR_026689 was determined and significantly increased in rat whole blood at the 10th and 20th week after NNK treatment to evaluate it as a potential early marker for lung cancer. Together, these findings suggest that lncRNA NR_026689 may be a potential early biomarker for lung cancer and is associated with lung carcinogenesis induced by NNK.

AB - Long non-coding RNAs (lncRNA) are thought to be important epigenetic regulators involved in the development of a variety of cancers. Alterations in lncRNA expression are associated with exposure to chemical carcinogens. However, it is still unclear whether lncRNA expression during lung carcinogenesis is induced by chemical carcinogens. In this study, using NNK-induced rat lung cancer model established by our previous study, we determined the lncRNA expression profiles, and an alteration in lncRNA expression was observed in lung cancer tissues and blood in the NNK treatment group. Using quantitative reverse-transcription PCR (qRT-PCR), five differentially expressed lncRNAs were further detected and validated. We identified a novel lncRNA, NR_026689, which showed increased expression in lung cancer tissues induced by NNK and the alteration of lncRNA NR_026689 was specifically observed in lung tissue. The level of NR_026689 was determined and significantly increased in rat whole blood at the 10th and 20th week after NNK treatment to evaluate it as a potential early marker for lung cancer. Together, these findings suggest that lncRNA NR_026689 may be a potential early biomarker for lung cancer and is associated with lung carcinogenesis induced by NNK.

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KW - NNK

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Identification of a long non-coding RNA NR_026689 associated with lung carcinogenesis induced by NNK

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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