Identification of AGE-modified proteins in SH-SY5Y and OLN-93 Cells

André K. Langer, H. Fai Poon, Gerald Münch, Bert C. Lynn, Thomas Arendt, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The formation of "Advanced Glycation End products" (AGEs) is an inevitable consequence of mammalian glucose metabolism. AGE-mediated protein-protein crosslinks lead to detergent-insoluble and protease-resistant protein aggregates, and in Alzheimer's disease (AD) extra cellular senile plaques (SPs) and intracellular neurofibrillary tangles (NFTs) have been shown to contain AGEs. However, to date little is known concerning the most prevalent protein-targets of AGE modification under normal, non-pathological conditions. Here, a combination of 2D-electrophoresis, Western blotting and mass spectrometry has been used to identify preferentially AGE-modified proteins in oligodendrocyte (OLN-93) and neuroblastoma cell lines (SH-SY5Y) in standard culture. Proteomics analysis identified a total of eight targets with structural, metabolic and regulatory function, three of which (β-actin, β-tubulin and eukaryotic Elongation Factor 1-α) were common to both cell lines. Based on results from prior studies, modification of these proteins may lead to a loss of function. Consequently, the identification of targets for these proteins is of particular interest for a better understanding of the consequences of AGE-modification in aging, neurodegenerative diseases and diabetes.

Original languageEnglish
Pages (from-to)255-268
Number of pages14
JournalNeurotoxicity Research
Volume9
Issue number4
DOIs
StatePublished - 2006

Bibliographical note

Funding Information:
This work was supported by the "Interdisziplnäre Zentrum für Klinische Forschung" (IZKF) Leipzig at the Faculty of Medicine of the University of Leipzig (01KS9504, project N1, and 961000-24), and by NIH grants to D.A.B..

Keywords

  • AGEs
  • Advanced glycation end products
  • Aging
  • Alzheimer disease
  • Diabetes
  • Eukaryotic elongation factor 1-α
  • Neurodecogenerative diseas
  • OLN-93cells
  • SH-SY5Y cells
  • β-abulin

ASJC Scopus subject areas

  • General Neuroscience
  • Toxicology

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