TY - JOUR
T1 - Identification of cross-linking site(s) of myosin heavy chains in oxidatively stressed chicken myofibrils
AU - Ooizumi, Tooru
AU - Xiong, Youling L.
PY - 2006/4
Y1 - 2006/4
N2 - The cross-linking site(s) of myosin heavy chains (MHC) in chicken myofibrils exposed to non-enzymatic, hydroxyl radical-generation oxidizing systems (HRGS) was investigated by means of chymotryptic digestion and subsequent electrophoresis. Oxidation of the chymotryptic digests resulted in cross-linking of the rod or light meromyosin (LMM) segment of MHC mostly via disulfide bonds, while subfragment-1 (S-1) or heavy meromyosin (HMM) was not affected. A mixture of cross-linked rod or LMM and uncross-linked S-1 or HMM was also produced when myofibrils were 1st oxidized and then digested with chymotrypsin, confirming that cross-linking of myosin in HRGS-oxidized myofibrils occurred initially in the LMM portion of the myosin rod.
AB - The cross-linking site(s) of myosin heavy chains (MHC) in chicken myofibrils exposed to non-enzymatic, hydroxyl radical-generation oxidizing systems (HRGS) was investigated by means of chymotryptic digestion and subsequent electrophoresis. Oxidation of the chymotryptic digests resulted in cross-linking of the rod or light meromyosin (LMM) segment of MHC mostly via disulfide bonds, while subfragment-1 (S-1) or heavy meromyosin (HMM) was not affected. A mixture of cross-linked rod or LMM and uncross-linked S-1 or HMM was also produced when myofibrils were 1st oxidized and then digested with chymotrypsin, confirming that cross-linking of myosin in HRGS-oxidized myofibrils occurred initially in the LMM portion of the myosin rod.
KW - Chymotryptic digestion
KW - Cross-linking
KW - Disulfide bond
KW - Myosin
KW - Oxidation
UR - http://www.scopus.com/inward/record.url?scp=33646381930&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646381930&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2621.2006.tb15617.x
DO - 10.1111/j.1365-2621.2006.tb15617.x
M3 - Article
AN - SCOPUS:33646381930
SN - 0022-1147
VL - 71
SP - C196-C199
JO - Journal of Food Science
JF - Journal of Food Science
IS - 3
ER -