Identification of differentially expressed thyroid hormone responsive genes from the brain of the Mexican Axolotl (Ambystoma mexicanum)

P. Huggins; C. K. Johnson; A. Schoergendorfer; S. Putta; A. C. Bathke; A. J. Stromberg; S. R. Voss

doi:10.1016/j.cbpc.2011.03.006

Identification of differentially expressed thyroid hormone responsive genes from the brain of the Mexican Axolotl (Ambystoma mexicanum)

P. Huggins, C. K. Johnson, A. Schoergendorfer, S. Putta, A. C. Bathke, A. J. Stromberg, S. R. Voss

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The Mexican axolotl (Ambystoma mexicanum) presents an excellent model to investigate mechanisms of brain development that are conserved among vertebrates. In particular, metamorphic changes of the brain can be induced in free-living aquatic juveniles and adults by simply adding thyroid hormone (T4) to rearing water. Whole brains were sampled from juvenile A. mexicanum that were exposed to 0, 8, and 18 days of 50 nM T4, and these were used to isolate RNA and make normalized cDNA libraries for 454 DNA sequencing. A total of 1,875,732 high quality cDNA reads were assembled with existing ESTs to obtain 5884 new contigs for human RefSeq protein models, and to develop a custom Affymetrix gene expression array (Amby-002) with approximately 20,000 probe sets. The Amby-002 array was used to identify 303 transcripts that differed statistically (p < 0.05, fold change > 1.5) as a function of days of T4 treatment. Further statistical analyses showed that Amby-002 performed concordantly in comparison to an existing, small format expression array. This study introduces a new A. mexicanum microarray resource for the community and the first lists of T4-responsive genes from the brain of a salamander amphibian.

Original language	English
Pages (from-to)	128-135
Number of pages	8
Journal	Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Volume	155
Issue number	1
DOIs	https://doi.org/10.1016/j.cbpc.2011.03.006
State	Published - Jan 2012

Bibliographical note

Funding Information:
We thank Robert Page and Meredith Boley for inducing metamorphosis in the A. mexicanum juveniles that were used in this experiment and for collecting brain tissues. The research was supported by grants R24-RR016344 and P20-RR16481 from the National Center for Research Resources (NCRR) , a component of the National Institutes of Health (NIH). The project also used resources developed under Multidisciplinary University Research Initiative grant ( W911NF-09-1-0305 ) from the Army Research Office , and resources from the Ambystoma Genetic Stock Center, which is funded by grant DBI-0951484 from the National Science Foundation . The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of NCRR, NIH, ARO, or NSF.

Keywords

Metamorphosis
Microarray
Salamander

ASJC Scopus subject areas

Biochemistry
Physiology
Aquatic Science
Animal Science and Zoology
Toxicology
Cell Biology
Health, Toxicology and Mutagenesis

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10.1016/j.cbpc.2011.03.006

Cite this

Huggins, P., Johnson, C. K., Schoergendorfer, A., Putta, S., Bathke, A. C., Stromberg, A. J., & Voss, S. R. (2012). Identification of differentially expressed thyroid hormone responsive genes from the brain of the Mexican Axolotl (Ambystoma mexicanum). Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 155(1), 128-135. https://doi.org/10.1016/j.cbpc.2011.03.006

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note = "Funding Information: We thank Robert Page and Meredith Boley for inducing metamorphosis in the A. mexicanum juveniles that were used in this experiment and for collecting brain tissues. The research was supported by grants R24-RR016344 and P20-RR16481 from the National Center for Research Resources (NCRR) , a component of the National Institutes of Health (NIH). The project also used resources developed under Multidisciplinary University Research Initiative grant ( W911NF-09-1-0305 ) from the Army Research Office , and resources from the Ambystoma Genetic Stock Center, which is funded by grant DBI-0951484 from the National Science Foundation . The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of NCRR, NIH, ARO, or NSF.",

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Huggins, P, Johnson, CK, Schoergendorfer, A, Putta, S, Bathke, AC, Stromberg, AJ & Voss, SR 2012, 'Identification of differentially expressed thyroid hormone responsive genes from the brain of the Mexican Axolotl (Ambystoma mexicanum)', Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, vol. 155, no. 1, pp. 128-135. https://doi.org/10.1016/j.cbpc.2011.03.006

Identification of differentially expressed thyroid hormone responsive genes from the brain of the Mexican Axolotl (Ambystoma mexicanum). / Huggins, P.; Johnson, C. K.; Schoergendorfer, A. et al.
In: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, Vol. 155, No. 1, 01.2012, p. 128-135.

Research output: Contribution to journal › Article › peer-review

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Identification of differentially expressed thyroid hormone responsive genes from the brain of the Mexican Axolotl (Ambystoma mexicanum)

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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