Identification of glucose-regulated miRNAs from pancreatic β cells reveals a role for miR-30d in insulin transcription

Xiaoqing Tang, Latha Muniappan, Guiliang Tang, Sabire Özcan

Research output: Contribution to journalArticlepeer-review

251 Scopus citations

Abstract

MicroRNAs (miRNAs) are small noncoding ribonucleotides that bind mRNAs and function mainly as translational repressors in mammals. MicroRNAs have been implicated to play a role in many diseases, including diabetes. Several reports indicate an important function for miRNAs in insulin production as well as insulin secretion. We have recently carried out a screen in the pancreatic β-cell line MIN6 to identify miRNAs with altered abundance in response to changes in glucose concentrations. This screen resulted in identification of 61 glucose-regulated miRNAs from a total of 108 miRNAs detectable in MIN6 cells. Many of the identified miRNAs, including miR-124a, miR-107, and miR-30d were up-regulated in the presence of high glucose. Only a few of the miRNAs, including miR-296, miR-484, and miR-690 were significantly down-regulated by high glucose treatment. Interestingly, we found that overexpression of miR-30d, one of the miRNAs up-regulated by glucose, increased insulin gene expression, while inhibition of miR-30d abolished glucose-stimulated insulin gene transcription. Overexpression or inhibition of miR-30d did not have any effect on insulin secretion. These data suggest that the putative target genes of miR-30d may be negative regulators of insulin gene expression. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalRNA
Volume15
Issue number2
DOIs
StatePublished - Feb 2009

Keywords

  • Diabetes
  • Glucose
  • MIN6
  • Pancreas
  • miR-30d
  • miRNA

ASJC Scopus subject areas

  • Molecular Biology

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